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- Rosetta（DE3）Rosetta(DE3)
- 大腸桿菌Rosetta（DE3）Escherichia coli Rosetta (DE3)
- Rosetta軟件Rosetta software
- Rosetta實(shí)驗系統在機器學(xué)習中的應用The Application of Rosetta in Machine Learning
- BL21（DE3）BI21 (DE3)
- 基于Rosetta軟件與Matlab6. 1完成了字符圖像的知識獲取。Knowledge acquisition based on the Rosetta and Matlab 6.1 software is accomplished.
- coliBL21(DE3)中.coli BL 21(DE 3).
- 大腸桿菌BL21（DE3）E. coli BL21 (DE3)
- 結論IL-24的重組載體在大腸桿菌BL21(DE3)可以表達GST-IL-24融合蛋白。The prokaryotic expression plasmid pGEX-IL-24 was constructed,GST-IL-24 molecular mass was approximately 50 000.Conclusion GST-IL-24 fusion protein was expressed in E. coli BL21(DE3) which was transformed from the recombinant vector of IL-24.
- 簡(jiǎn)要介紹了粗糙集理論的基本原理和主要方法,給出了一個(gè)基于粗糙集理論設計的Rosetta軟件對心臟病進(jìn)行診斷的應用實(shí)例。This paper briefly introduces the principles and methods of rough set theory,and gives an example applying Rosetta software based on rough set theory to diagnose the heart disease.
- coliBL21(DE3)作為表達宿主菌。coli BL21(DE3) cell was chosen as the host cell for the expression of the fusion protein.
- coliBL21(DE3)/pRY1 中,nanA 基因受lac啟動(dòng)子控制,為IPTG 或乳糖所誘導表達。Furthermore,the plasmid pRY3 was constructed,in which the nanA gene is controlled by the tac promoter .
- 本文介紹粗糙集理論的基本概念 ,運用 VPRS模型對車(chē)險保單數據進(jìn)行分析 ,借助基于粗糙集理論的數據挖掘工具 ROSETTA挖掘出隱含在其中的的風(fēng)險規則Then it establishes a mining model for the insurance risk rules based on VPRS Rough set. Finally, with the tool ROSETTA based on Rough set, the model was applied to extract risk rules from the database of customers, and so some rules are given.
- 大腸桿菌BL21(DE3)lpxM突變株的構建Construction of a Mutant E.coli BL21(DE3) with Inactivated lpxM
- 用多個(gè)UCI數據集對算法進(jìn)行了測試，并且與著(zhù)名的Rosetta軟件進(jìn)行實(shí)驗對比，結果說(shuō)明此方法大大提高了總的數據約簡(jiǎn)量，可以有效地簡(jiǎn)化最終得到的規則知識。The paper also uses many UCI data sets to test the proposed approach and compares with the Rossetta tool. The results show this method greatly improves the total data reduction and efficiently simplifies the rule sets.
- 用IPTG誘導,LG3蛋白在大腸桿菌BL21(DE3)中得到了表達,并經(jīng)Ni-NTA層析柱獲得純化。The recombinant plasmid was transformed into E. coli BL21(DE3), and E. coli BL21(DE3)/pET-LG3 was induced by IPTG.
- 大腸桿菌BL21(DE3)中表達重組蛋白的研究The expression analysis of recombinant protein in \\%25Escherichia coli BL21 (DE3).
- 將pET-32CAR轉化大腸桿菌BL21（DE3）。coil BL21(DE3), and 1mM IPTG wasused to induce the fusion gene to expression.
- 將重組表達載體在大腸桿菌BL21(DE3)中以1mol/L IPTG 誘導4h 或者更長(cháng)時(shí)間進(jìn)行表達。Coli BL21(DE3) was introduced by 1mmol/L IPTG for 4h or more time for expression.
- 經(jīng)酶切鑒定和序列測定后，將pET-scFv轉化入大腸桿菌BL21star(DE3)中，進(jìn)行誘導表達。coli BL21star (DE3) and expression of the recombinant scFv gene was induced by addition of 0.2 mM IPTG.
