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- 結論IL-24的重組載體在大腸桿菌BL21(DE3)可以表達GST-IL-24融合蛋白。The prokaryotic expression plasmid pGEX-IL-24 was constructed,GST-IL-24 molecular mass was approximately 50 000.Conclusion GST-IL-24 fusion protein was expressed in E. coli BL21(DE3) which was transformed from the recombinant vector of IL-24.
- 將重組表達載體在大腸桿菌BL21(DE3)中以1mol/L IPTG 誘導4h 或者更長(cháng)時(shí)間進(jìn)行表達。Coli BL21(DE3) was introduced by 1mmol/L IPTG for 4h or more time for expression.
- 大腸桿菌BL21（DE3）E. coli BL21 (DE3)
- 利用表達載體pET-22b(+),實(shí)現了色褐鏈霉菌磷脂酶D基因在大腸桿菌BL21(DE3)中的高效表達。In this research,the gene encoding phospholipase D from Streptomyces chromofuscus was cloned into expressed vector pET-22b(+) constituting recombinant plasmid pET-pld which was tranformed into E. coli BL21(DE3).
- 將重組質(zhì)粒轉化到大腸桿菌BL21(DE3)中進(jìn)行表達,表達的核蛋白再經(jīng)Ni2+-NTA親和層析純化。Then they were transformed into Escherichia coli BL21(DE3) competent cells for expression. The expressed nucleoprotein was purified using Ni2+-NTA Column under denaturing conditions.
- P185~(c-erBb-2)胞外區結構域在大腸桿菌BL21中的表達分析The Expression Analysis of Extracellular Domain of P185~( c-erBb-2) in E.coli (BL21)
- 大腸埃希氏菌, 大腸桿菌Escherichia coli
- 將PET30bVP2和rBacVP2分別在大腸桿菌BL21株和sf9昆蟲(chóng)細胞中進(jìn)行表達。coli BL21 strain and sf9 insect cell.
- 大腸桿菌含量level of Escherichia coli
- 大腸桿菌PGDH末端缺失突變體的構建及抗反饋抑制效應分析Construction and Characterization of E. Coli PGDH Mutants with Feedback-inhibition Resistance
- 鴨大腸桿菌pathogenic Escherichia coli from duck
- 將gfpxm克隆至pTO-T7表達載體，GFPxm在大腸桿菌BL21中的表達量達菌體總蛋白的50%左右。The entire coding sequence was cloned into the pTO-T7 expression vector and expressed in E coli BL21. The expression yield of GFPxm was amounted to 50%25 of the total protein. Compared with GFP of A.
- 禽大腸桿菌APEC
- 大腸桿菌K12E. Coli K12
- 大腸桿菌O85E. coli O85
- 重組大腸桿菌BL21recombinant
- F18大腸桿菌Escherichia coli F18
- 大腸桿菌F18Escherichia coli F18
- BL21（DE3）BI21 (DE3)
- 兔大腸桿菌rabbit E. coli
