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- 將重組表達載體在大腸桿菌BL21(DE3)中以1mol/L IPTG 誘導4h 或者更長(cháng)時(shí)間進(jìn)行表達。Coli BL21(DE3) was introduced by 1mmol/L IPTG for 4h or more time for expression.
- 利用表達載體pET-22b(+),實(shí)現了色褐鏈霉菌磷脂酶D基因在大腸桿菌BL21(DE3)中的高效表達。In this research,the gene encoding phospholipase D from Streptomyces chromofuscus was cloned into expressed vector pET-22b(+) constituting recombinant plasmid pET-pld which was tranformed into E. coli BL21(DE3).
- 將重組質(zhì)粒轉化到大腸桿菌BL21(DE3)中進(jìn)行表達,表達的核蛋白再經(jīng)Ni2+-NTA親和層析純化。Then they were transformed into Escherichia coli BL21(DE3) competent cells for expression. The expressed nucleoprotein was purified using Ni2+-NTA Column under denaturing conditions.
- BL21（DE3）BI21 (DE3)
- P185~(c-erBb-2)胞外區結構域在大腸桿菌BL21中的表達分析The Expression Analysis of Extracellular Domain of P185~( c-erBb-2) in E.coli (BL21)
- Rosetta（DE3）Rosetta(DE3)
- coliBL21(DE3)中.coli BL 21(DE 3).
- 重組大腸桿菌BL21recombinant
- 大腸桿菌Rosetta（DE3）Escherichia coli Rosetta (DE3)
- 將PET30bVP2和rBacVP2分別在大腸桿菌BL21株和sf9昆蟲(chóng)細胞中進(jìn)行表達。coli BL21 strain and sf9 insect cell.
- 目的優(yōu)化BL21/pET-11 c/hIL-2-mGM-CSF的培養條件以提高目的蛋白的表達量。Objective To optimize the culture condition of engineered E. coli to improve its expression efficiency of hIL-2-mGM-CSF protein.
- coliBL21(DE3)作為表達宿主菌。coli BL21(DE3) cell was chosen as the host cell for the expression of the fusion protein.
- coliBL21(DE3)/pRY1 中,nanA 基因受lac啟動(dòng)子控制,為IPTG 或乳糖所誘導表達。Furthermore,the plasmid pRY3 was constructed,in which the nanA gene is controlled by the tac promoter .
- 岸蟹金屬硫蛋白在大腸桿菌BL21中的表達Expression of Carcinus maenas Metallothionein Gene in Escherichia Coli BL21
- 經(jīng)酶切鑒定和序列測定后，將pET-scFv轉化入大腸桿菌BL21star(DE3)中，進(jìn)行誘導表達。coli BL21star (DE3) and expression of the recombinant scFv gene was induced by addition of 0.2 mM IPTG.
- 將gfpxm克隆至pTO-T7表達載體，GFPxm在大腸桿菌BL21中的表達量達菌體總蛋白的50%左右。The entire coding sequence was cloned into the pTO-T7 expression vector and expressed in E coli BL21. The expression yield of GFPxm was amounted to 50%25 of the total protein. Compared with GFP of A.
- 皮蠅素HA基因的克隆和在大腸桿菌BL21中的表達Cloning and Expression of Hypodermin A Gene in BL21E.coli
- 人細胞周期蛋白D1在大腸桿菌BL21中的表達及純化Expression and Purification of Recombinant Human CyclinD1 in E.coli BL21
- coliRosetta(DE3)中獲得高效表達,SDS-PAGE分析表明表達產(chǎn)物大部分以包涵體形式存在。coli Rosetta(DE3) and was mainly present in inclusion body observed by SDS-PAGE.
- 小鼠canstatin及其N(xiāo)端片段在大腸桿菌BL21中的表達Expression of Mouse canstatin and its N-domain in E.coli BL21
