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- 方法以常規克隆方法將目的片段連接至pMAL-p2x載體,獲得的陽(yáng)性克隆轉化大腸桿菌Rosetta(DE3)進(jìn)行表達。coli Rosetta(DE3) as a maltose-binding protein(MBP) fusion protein using the pMAL-p2x expression system.
- 大腸桿菌Rosetta（DE3）Escherichia coli Rosetta (DE3)
- Rosetta（DE3）Rosetta(DE3)
- 結論IL-24的重組載體在大腸桿菌BL21(DE3)可以表達GST-IL-24融合蛋白。The prokaryotic expression plasmid pGEX-IL-24 was constructed,GST-IL-24 molecular mass was approximately 50 000.Conclusion GST-IL-24 fusion protein was expressed in E. coli BL21(DE3) which was transformed from the recombinant vector of IL-24.
- Rosetta軟件Rosetta software
- Rosetta實(shí)驗系統在機器學(xué)習中的應用The Application of Rosetta in Machine Learning
- 大腸埃希氏菌, 大腸桿菌Escherichia coli
- 大腸桿菌含量level of Escherichia coli
- 基于Rosetta軟件與Matlab6. 1完成了字符圖像的知識獲取。Knowledge acquisition based on the Rosetta and Matlab 6.1 software is accomplished.
- 大腸桿菌PGDH末端缺失突變體的構建及抗反饋抑制效應分析Construction and Characterization of E. Coli PGDH Mutants with Feedback-inhibition Resistance
- BL21（DE3）BI21 (DE3)
- 鴨大腸桿菌pathogenic Escherichia coli from duck
- coliBL21(DE3)中.coli BL 21(DE 3).
- 禽大腸桿菌APEC
- 大腸桿菌K12E. Coli K12
- 大腸桿菌O85E. coli O85
- F18大腸桿菌Escherichia coli F18
- 大腸桿菌F18Escherichia coli F18
- 兔大腸桿菌rabbit E. coli
- 以質(zhì)粒pCWNF14為模板,采用PCR技術(shù)擴增出CYP3A4基因,并將其接入pET-22b(+)、pET-28b(+)和pET-32a(+)載體中,經(jīng)PCR測序鑒定后,轉化Rosetta(DE3)2pLysS細菌,并用IPTG誘導表達.The CYP3A4 gene was amplified by method of PCR technique from the template of pCWNF14 plasmid,then was cloned into pET-22b(+),pET-28b(+),and pET-32a(+)vector and identified with PCR and sequencing. The recombinant expression plasmid containing CYP3A4 gene was transformed into Rosetta(DE3)2pLysS and target protein expression was induced by IPTG.
