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- METHODS: The HAb18GEF gene fragment was amplified by PCR and digested with Sac I/Not I,and then cloned into the multiple clone site downstream of EpoR/LR-F3 gene of eukaryotic expression vector pCEL2f. 方法:用PCR法擴增肝癌相關(guān)抗原HAb18G胞外段基因,SacI/NotI雙酶切后插入真核表達載體pCEL2f中,構建EpoR/LR-F3/HAb18GEF嵌合受體基因的重組表達載體pCEL2f/HAb18GEF,并經(jīng)酶切和測序驗證。
- Methods The full length of CD95 cDNA was inserted into the multiple cloning site of the expression vector pBK CMB by molecular cloning technique. 方法 采用分子克隆技術(shù)將CD95基因插入真核表達載體pBK CMV的多克隆克隆位點(diǎn)之間 ,以脂質(zhì)體介導法將目的基因導入受體細胞SGC790 1,用G418篩選克隆細胞 ;
- multiple clone site 多克隆位點(diǎn)
- The products of PCR werenamed Sa and Sb, of 2.1Kb and 2.3Kb respectively. Sa was inserted in EcoR I andKpn I sites after Sb was cloned in KpnI and PstI multiple cloning sites of the samepUC18 plasmid. 1Kb和2.;3Kb兩個(gè)片段，經(jīng)酶切鑒定后，先后插入到pUC18質(zhì)粒載體上的EcoRI和PstI多克隆位點(diǎn)上，構建了重組質(zhì)粒pUC-S。
- The gene segments mentioned above were cloned into the multiple cloning sites of retrovirus vector pLXSN to construct an attenuated superantigen expression vector pLXSN SEA (D227A) linker CD80tm modulated by cis acting element of AFP gene. 將上述片斷插入逆轉錄病毒載體 pLXSN的多克隆位點(diǎn) ,構建AFP基因順式作用元件調控的肝癌特異性減毒超抗原表達載體 (pLXSNSEA(D2 2 7A) linker CD80tm)。
- multiple cloning site 多克隆位點(diǎn)
- The first item: monoclone antibody and multiple clone antibody have exerted abroad in biology research, clinic diagnosisand therapy field.It"s a huge market. 其中即將上馬的第一個(gè)項目：?jiǎn)慰寺】贵w和多克隆抗體在生物學(xué)研究、臨床診斷和治療等領(lǐng)域中已被廣泛運用，具有非常廣闊的市場(chǎng)前景。
- multiple cloning sites 多克隆位點(diǎn)(分)
- While most of Baker's clone characters are recorded separately, he sometimes voices multiple clones at once, depending on the complexity of the dialogue and the number of clones in the scene. 雖然絕大多數貝克的克隆人角色都是分別錄音的，但他有時(shí)也要根據對話(huà)的復雜程度以及場(chǎng)景中的克隆人數，一次配多個(gè)克隆人。
- Methods: A cDNA fragment from the mRNAs of skeletal muscle of 13 day old chicken embryo was amplified by RT PCR method, and inserted into pGEM and pUC vectors at clone sites and sequenced respectively. 方法 :從 13d雞胚骨骼肌mRNAs經(jīng)RT PCR方法擴增RTEF 1的編碼序列 (cDNA) ,將其克隆到 pGEM和 pUC載體并進(jìn)行核苷酸序列分析。
- The TuMV isolate CDN1 infectious full-length cDNA clone pVIR95T was modified into an expression vector pVIR95TM by inserting the adaptor with the MCS and the new NIa recognition site after the site between the Nib and CP by PCR. 在此基礎上，將含有多克隆位點(diǎn)和編碼NIa蛋白酶識別位點(diǎn)的Adaptor通過(guò)PCR介導插入到蕪菁花葉病毒CDN1株系全長(cháng)cDNA的NIb與CP區之間的NIa蛋白酶識別位點(diǎn)之后，將蕪菁花葉病毒CDN1株系全長(cháng)cDNA侵染性克隆pVIR95T改造成一個(gè)可利用的基因插入型表達載體pVIR95TM。
- In the end, an expression T vector pBV-HX, which is the first practical use of the HphI-XcmI linker as a universal cloning site, was constructed by using the linker insert into vector pBV220. 將HphI-XcmI接頭接入pBV220中構成一表達T載體是該接頭的第一個(gè)實(shí)際應用。
- He is the person with multiple injuries. 他就是那個(gè)多處受傷的人。
- Friendship multiple joy and divide grieve. 朋友之間，歡樂(lè )同享，苦難同當。
- We use the hall for multiple purposes. 這個(gè)大廳我們派多種用途。
- Wait for foster mother to give birth to the clone. 等待“養母”產(chǎn)下克隆幼仔。
- Will 2003 be the year of the first human clone? 人類(lèi)第一個(gè)克隆人會(huì )在2003年誕生嗎?
- The first clone farm has appeared in New Zealand. 第一家克隆農場(chǎng)在新西蘭出現。
- A multiple crash can often be seen on that motorway. 那條公路上的聯(lián)環(huán)撞車(chē)事故很多。
- Making Vehicle Digital Instruments based on MCS 51 MCU. 基于51單片機的車(chē)用數字儀表設計與實(shí)現。
