The TuMV isolate CDN1 infectious full-length cDNA clone pVIR95T was modified into an expression vector pVIR95TM by inserting the adaptor with the MCS and the new NIa recognition site after the site between the Nib and CP by PCR.
英
美
- 在此基礎上，將含有多克隆位點(diǎn)和編碼NIa蛋白酶識別位點(diǎn)的Adaptor通過(guò)PCR介導插入到蕪菁花葉病毒CDN1株系全長(cháng)cDNA的NIb與CP區之間的NIa蛋白酶識別位點(diǎn)之后，將蕪菁花葉病毒CDN1株系全長(cháng)cDNA侵染性克隆pVIR95T改造成一個(gè)可利用的基因插入型表達載體pVIR95TM。
