Methods: A cDNA fragment from the mRNAs of skeletal muscle of 13 day old chicken embryo was amplified by RT PCR method, and inserted into pGEM and pUC vectors at clone sites and sequenced respectively.

 
  • 方法 :從 13d雞胚骨骼肌mRNAs經(jīng)RT PCR方法擴增RTEF 1的編碼序列 (cDNA) ,將其克隆到 pGEM和 pUC載體并進(jìn)行核苷酸序列分析。
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