METHODS: The HAb18GEF gene fragment was amplified by PCR and digested with Sac I/Not I,and then cloned into the multiple clone site downstream of EpoR/LR-F3 gene of eukaryotic expression vector pCEL2f.
英
美
- 方法:用PCR法擴增肝癌相關(guān)抗原HAb18G胞外段基因,SacI/NotI雙酶切后插入真核表達載體pCEL2f中,構建EpoR/LR-F3/HAb18GEF嵌合受體基因的重組表達載體pCEL2f/HAb18GEF,并經(jīng)酶切和測序驗證。
