The gene segments mentioned above were cloned into the multiple cloning sites of retrovirus vector pLXSN to construct an attenuated superantigen expression vector pLXSN SEA (D227A) linker CD80tm modulated by cis acting element of AFP gene.

 
  • 將上述片斷插入逆轉錄病毒載體 pLXSN的多克隆位點(diǎn) ,構建AFP基因順式作用元件調控的肝癌特異性減毒超抗原表達載體 (pLXSNSEA(D2 2 7A) linker CD80tm)。
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