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- Finally, the linear pBLI plasmid vector coated with liposomere agent,was transferred into the bFF to validate pBLI. 最后,以線(xiàn)性化的乳腺特異表達載體pBLI為外源基因,采用脂質(zhì)體介導法轉染牛胎兒成纖維細胞。
- The researchers then used a plasmid vector to transiently increase levels of oct4 in hESC. 科學(xué)家們利用基因抑制技術(shù)（knockdown）來(lái)降低oct4基因的表達，這是一種對老鼠和人類(lèi)中胚胎干細胞進(jìn)行自我更新非常關(guān)鍵的基因。
- In addition, a set of expression plasmid vectors, PMS-31b. 同時(shí)構建一組質(zhì)粒表達載體PMS-31b。
- We applied real-time image and fluorescence recovery after photobleaching (FRAP) to examine the nuclear pore permeability of 27 kDa EGFP in human lung cancer cells (ASTC-a-1) transfected with plasmid vector (pEGFP-C1). 本實(shí)驗利用熒光漂白后恢復(FRAP,fluorescencerecoveryafterphotobleaching)法觀(guān)測人肺腺癌腫瘤細胞(ASTC-a-1)中表達的27kDaEGFP在核質(zhì)間的被動(dòng)擴散,并以激光共焦顯微鏡進(jìn)行實(shí)時(shí)成像。
- The recombinant Herpesviruses of Turkey transfer plasmid vector pT-C-gB-IL was cotransfected with Herpesviruses of Turkey at CEF by calcium phosphate-DNA coprecipitation method and the target fragment was inserted into TK domain of HVT. 確定轉染時(shí)親本毒的用量,用磷酸鈣共沉淀法將HVT轉移質(zhì)粒載體pT-C-gB-IL與HVT在CEF細胞上進(jìn)行同源重組,將目的基因導入HVT TK區。
- METHODS: Bcl-XL siRNA and negative siRNA plasmid vector were stably transfected into A549/DDP cells. Drug sensitivity of the cells to cisplatin(DDP) was analyzed with MTT and flow cytometry. 方法:將Bcl-XLsiRNA質(zhì)粒載體轉染至A549/DDP細胞,MTT、流式細胞儀檢測細胞藥物敏感性的改變;
- The human colon cancer LoVo cells were transfected by Lipofectamine 2000 with plasmid vector, and then selected for 4 weeks by G418. The expression of cerb-B1 mRNA was assessed by real time PCR and the protein of EGFR was assessed by Western blot method. 應用脂質(zhì)體Lipofectamine2000轉染人結腸癌LoVo細胞,轉染成功后以G418篩選4周,實(shí)時(shí)熒光定量RT-PCR(RealTimeRT-PCR)檢測cerb-B1基因的mRNA表達,Western-blot檢測EGFR蛋白的表達。
- The Situation of Plasmid Vector in Gene Therapy 以質(zhì)粒為載體的基因治療現狀
- Plasmid Vector Suitable for Gene Therapy or Immunization 一種基因治療和基因免疫表達載體的構建
- THE APPLICATION OF PCR AMPLIFICATION DIGESTED WITH ENDONUCLEASE OF YEAST PLASMID IN YEAST TWO-HYBRID EXPERIMENTS 酵母質(zhì)粒PCR擴增及酶切在酵母雙雜交中的運用
- CONSTRUCTION OF SOIL METEGENOMIC LIBRARY WITH PLASMID VECTOR 以質(zhì)粒載體構建土壤宏基因組文庫的研究
- Yeast must be used in producing wine. 制酒時(shí)必須使用酵母菌。
- Human lung cancer cells (ASTC a 1) were infected with EGFP plasmid vectors, and incubated in RPMI 1640 culture medium supplemented with 15% FCS and 800 mg/L G418. Strong fluorescence of five cell clones were observed after several generations. 以綠色熒光蛋白 (GFP)作為標記基因轉入人類(lèi)肺癌細胞系 (ASTC a 1) ;經(jīng) 80 0mg/LG4 18篩選 ;獲得5株高表達細胞系 .
- Yeast can be used in making beer and bread. 酵母可用于釀啤酒和發(fā)面包。
- In this paper, the research in optimization design for DNA vaccine was reviewed, including choice of antigen gene and immune adjuvant, optimization of plasmid vectors and inoculation methods. 許多試圖增強和調節質(zhì)粒DNA疫苗的免疫原性和效率的方案都在研究之中。
- Secondly, we selected yeast Pichia pastoris to be the gene-expressing host and the secreting plasmid pPIC9 was as vector to construct recombined plasmid pPIC-Atmp. 研究中，我們選用巴斯德畢赤酵母作為基因表達系統，分泌型質(zhì)粒pPIC9作為載體，構建了基因工程菌。
- Made light and high by yeast or other leaven. 發(fā)酵的用酵母或其它發(fā)酵劑使 ... 輕而高
- CONSTRUCTION OF NOS/HuIFNa CHIMERIC GENE AND ITS FUSION WITH Ti PLASMID VECTOR NOS/HuIFNa嵌合基因的構建及其與Ti質(zhì)粒載體的拼接
- A novel shuttle plasmid which can be replicated in yeast and expressed in mammal cell is required in oral gene therapy with yeast as vector to deliver target gene. 為研究酵母作為載體在口服基因治療及免疫中的作用,需要一種能夠在酵母中復制而在哺乳動(dòng)物細胞中表達的穿梭載體。
- The Construction of Shuttle Plasmid Vector of Streptomyces and E. colt 鏈霉菌和大腸桿菌穿梭質(zhì)粒載體的構建
