The human colon cancer LoVo cells were transfected by Lipofectamine 2000 with plasmid vector, and then selected for 4 weeks by G418. The expression of cerb-B1 mRNA was assessed by real time PCR and the protein of EGFR was assessed by Western blot method.
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- 應用脂質(zhì)體Lipofectamine2000轉染人結腸癌LoVo細胞,轉染成功后以G418篩選4周,實(shí)時(shí)熒光定量RT-PCR(RealTimeRT-PCR)檢測cerb-B1基因的mRNA表達,Western-blot檢測EGFR蛋白的表達。
