We applied real-time image and fluorescence recovery after photobleaching (FRAP) to examine the nuclear pore permeability of 27 kDa EGFP in human lung cancer cells (ASTC-a-1) transfected with plasmid vector (pEGFP-C1).
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- 本實(shí)驗利用熒光漂白后恢復(FRAP,fluorescencerecoveryafterphotobleaching)法觀(guān)測人肺腺癌腫瘤細胞(ASTC-a-1)中表達的27kDaEGFP在核質(zhì)間的被動(dòng)擴散,并以激光共焦顯微鏡進(jìn)行實(shí)時(shí)成像。
