The recombinant Herpesviruses of Turkey transfer plasmid vector pT-C-gB-IL was cotransfected with Herpesviruses of Turkey at CEF by calcium phosphate-DNA coprecipitation method and the target fragment was inserted into TK domain of HVT.

 
  • 確定轉染時(shí)親本毒的用量,用磷酸鈣共沉淀法將HVT轉移質(zhì)粒載體pT-C-gB-IL與HVT在CEF細胞上進(jìn)行同源重組,將目的基因導入HVT TK區。
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