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- Finally, the linear pBLI plasmid vector coated with liposomere agent,was transferred into the bFF to validate pBLI. 最后,以線(xiàn)性化的乳腺特異表達載體pBLI為外源基因,采用脂質(zhì)體介導法轉染牛胎兒成纖維細胞。
- The researchers then used a plasmid vector to transiently increase levels of oct4 in hESC. 科學(xué)家們利用基因抑制技術(shù)（knockdown）來(lái)降低oct4基因的表達，這是一種對老鼠和人類(lèi)中胚胎干細胞進(jìn)行自我更新非常關(guān)鍵的基因。
- Coincidentally, the psoriasis paper mentioned a recent study that linked SLE to low copy number of complement C4 genes. 巧合的是，銀屑病論文提到近期一項研究將SLE與補體蛋白C4基因拷貝數低聯(lián)系起來(lái)。
- Conclusion: The method combined DOP-PCR with CGH can realize the cytogenetic analysis of the human genome from low copy number, and the analysis result is accurate and reliable. 結論：本研究所建立的單細胞全基因組擴增聯(lián)合比較基因組雜交技術(shù)適用于單細胞等低拷貝樣本的分析，分析結果準確、可靠。
- In addition, a set of expression plasmid vectors, PMS-31b. 同時(shí)構建一組質(zhì)粒表達載體PMS-31b。
- We applied real-time image and fluorescence recovery after photobleaching (FRAP) to examine the nuclear pore permeability of 27 kDa EGFP in human lung cancer cells (ASTC-a-1) transfected with plasmid vector (pEGFP-C1). 本實(shí)驗利用熒光漂白后恢復(FRAP,fluorescencerecoveryafterphotobleaching)法觀(guān)測人肺腺癌腫瘤細胞(ASTC-a-1)中表達的27kDaEGFP在核質(zhì)間的被動(dòng)擴散,并以激光共焦顯微鏡進(jìn)行實(shí)時(shí)成像。
- The recombinant Herpesviruses of Turkey transfer plasmid vector pT-C-gB-IL was cotransfected with Herpesviruses of Turkey at CEF by calcium phosphate-DNA coprecipitation method and the target fragment was inserted into TK domain of HVT. 確定轉染時(shí)親本毒的用量,用磷酸鈣共沉淀法將HVT轉移質(zhì)粒載體pT-C-gB-IL與HVT在CEF細胞上進(jìn)行同源重組,將目的基因導入HVT TK區。
- METHODS: Bcl-XL siRNA and negative siRNA plasmid vector were stably transfected into A549/DDP cells. Drug sensitivity of the cells to cisplatin(DDP) was analyzed with MTT and flow cytometry. 方法:將Bcl-XLsiRNA質(zhì)粒載體轉染至A549/DDP細胞,MTT、流式細胞儀檢測細胞藥物敏感性的改變;
- The rate of low copy number of CATT repeat (less or equal to 6) was significantly higher in lung tuberculosis patients than in normal people(85.6% vs 66.6%),suggesting that MIF might play a reciprocity in the pathogenesis of tuberculosis. 對比分析提示;肺結核病人MIF基因啟動(dòng)子區CATT低拷貝數(低于或等于6個(gè))出現的頻率明顯高于正常人(85.;6%25比66
- The human colon cancer LoVo cells were transfected by Lipofectamine 2000 with plasmid vector, and then selected for 4 weeks by G418. The expression of cerb-B1 mRNA was assessed by real time PCR and the protein of EGFR was assessed by Western blot method. 應用脂質(zhì)體Lipofectamine2000轉染人結腸癌LoVo細胞,轉染成功后以G418篩選4周,實(shí)時(shí)熒光定量RT-PCR(RealTimeRT-PCR)檢測cerb-B1基因的mRNA表達,Western-blot檢測EGFR蛋白的表達。
- The Situation of Plasmid Vector in Gene Therapy 以質(zhì)粒為載體的基因治療現狀
- Construction of a low molecular weight plasmid vector for the capture of genes encoding exported proteins 捕獲輸出蛋白編碼基因的小分子量質(zhì)粒載體的構建與驗證
- Plasmid Vector Suitable for Gene Therapy or Immunization 一種基因治療和基因免疫表達載體的構建
- CONSTRUCTION OF SOIL METEGENOMIC LIBRARY WITH PLASMID VECTOR 以質(zhì)粒載體構建土壤宏基因組文庫的研究
- The government is trying to keep a low profile on this issue. 政府力圖在這個(gè)問(wèn)題上保持低姿態(tài)。
- The singer forced his low notes. 那位歌手勉強唱出低音。
- Human lung cancer cells (ASTC a 1) were infected with EGFP plasmid vectors, and incubated in RPMI 1640 culture medium supplemented with 15% FCS and 800 mg/L G418. Strong fluorescence of five cell clones were observed after several generations. 以綠色熒光蛋白 (GFP)作為標記基因轉入人類(lèi)肺癌細胞系 (ASTC a 1) ;經(jīng) 80 0mg/LG4 18篩選 ;獲得5株高表達細胞系 .
- In this paper, the research in optimization design for DNA vaccine was reviewed, including choice of antigen gene and immune adjuvant, optimization of plasmid vectors and inoculation methods. 許多試圖增強和調節質(zhì)粒DNA疫苗的免疫原性和效率的方案都在研究之中。
- He because of bashful but low head. 他因羞愧而低下了頭。
- The ship lay keeled over at low tide. 那艘船因退潮而傾覆。
