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- Use RT-PCR and real time PCR to quantify the HLA-G mRNA on deciduas. RT-PCR和real time PCR法檢測胎盤(pán)蛻膜組織HLA-G mRNA。
- The plasma biochemical indexes were detected by using the kit, and real time PCR was used to analyze the expression of adipogenesis genes with time series. 試劑盒檢測血脂生化指標，熒光定量 PCR 技術(shù)檢測聚脂相關(guān)基因的轉錄表達規律。
- Totally 240 throat swab specimens collected from adults with flu like symptoms were detected both by the multiplex real time PCR system and conventional virus isolation. 我們收集240名成人具有類(lèi)流感癥狀的咽喉拭子檢體，利用此技術(shù)進(jìn)行病毒篩檢，并與病毒培養結果比較。
- The population of M. formicicum by real time PCR was lower than the population of M. formicicum by the method of most probable number, and the positive correlation between them was very high(r=0.957. P<0.01). M.;formicicum采用Real Time PCR方法測得的數量低于采用傳統最大或然數法測得的數量;但兩者之間具有很高的相關(guān)性(r=0
- Methods A549 cells were treated with different cytotoxic drugs for 24hours. The changes of APE/ref-1 expression in A549 after drug-treatment were detected by real time PCR, western blot and streptavidin peroxidase immunohistochemistry staining. 方法 通過(guò)免疫組化染色明確APE/ref-1在A(yíng)549細胞中的表達后,以特定濃度的臨床常用細胞毒性藥物對A549細胞進(jìn)行誘導,采用實(shí)時(shí)熒光定量PCR、Western Blot和免疫組化技術(shù)檢測藥物誘導前后A549細胞內APE/ref-1表達的變化情況。
- Genehunter analysis is concordant with ETDT. By real time PCR, OAZ(OLF1/ EBF associated zinc finger protein)gene which is located in the positive locus showed higher expression in SLE patients than in normal controls. 對候選基因的研究顯示 OAZ(OL F1/ EBF- associated zinc finger protein)基因表達顯著(zhù)高于正常對照。
- Methods Quantitative detection of T cell receptor excision DNA circles (TRECs) in DNA of peripheral blood mononuclear cells from 8 cases with CML, CD4 + or CD8 + cells from 3 cases with CML were preformed by real time PCR (TaqMan) analysis. 方法 利用實(shí)時(shí)定量PCR(Taq Man)方法檢測 8例CML患者外周血單個(gè)核細胞、3例CML患者CD4 + 和CD8+ 細胞中TRECs的水平。
- The human colon cancer LoVo cells were transfected by Lipofectamine 2000 with plasmid vector, and then selected for 4 weeks by G418. The expression of cerb-B1 mRNA was assessed by real time PCR and the protein of EGFR was assessed by Western blot method. 應用脂質(zhì)體Lipofectamine2000轉染人結腸癌LoVo細胞,轉染成功后以G418篩選4周,實(shí)時(shí)熒光定量RT-PCR(RealTimeRT-PCR)檢測cerb-B1基因的mRNA表達,Western-blot檢測EGFR蛋白的表達。
- RT-PCR, real time PCR or NASBA are used to pathogen test on cloacal or throat swab and wild birds.If the conformity rate >70% for HI antibody, it can be certified as conformity of vaccination. 疫情監測用RT-PCR或熒光定量PCR或NASBA(以來(lái)核酸序列的擴增技術(shù))等方法進(jìn)行泄殖腔拭子、咽喉拭子和野鳥(niǎo)糞便的病原學(xué)檢測。
- In order to to detect Penicillium expansum rapidly in apple juice concentration,the real time PCR method was used to detect Penicillium expansum and the conditions were also optimized. 為了研究蘋(píng)果汁生產(chǎn)中棒曲霉素產(chǎn)生菌的快速檢測,利用實(shí)時(shí)PCR方法快速檢測擴展青霉的可能性,對擴增條件進(jìn)行優(yōu)化。
- Lee, D. S., “Reserch and Development of a Novel Real Time PCR Machine,” Department of Mechanical Engineering, National Taiwan University, Dissertation for Doctor of Philosophy, 2004. 吳孟勛，新型復式即時(shí)定量聚合酶連鎖反應檢測機臺開(kāi)發(fā)與研究，國立臺灣大學(xué)機械工程研究所碩士論文，臺北，2004。
- Real time PCR quantification methods 實(shí)時(shí)定量法
- Keywords hepatitis B virus;real time PCR;HBeAg; 乙型肝炎病毒;聚合酶鏈反應;熒光定量;
- Keywords Real Time PCR;virus;Inactivation and removal efficiency; 實(shí)時(shí)熒光定量PCR;病毒;滅活去除效率;
- Keywords Real time PCR;Rumen microorganisms;Quantification;Plant oil;Methane; 瘤胃微生物;定量;植物油;甲烷;
- This will be very quick. It will be real time. 它將是非?？旖莺蛯?shí)時(shí)的。
- PCR and Real Time PCR Detection for Enterobacter sakazakii in Dehydrated Powdered Milk 奶粉中阪崎腸桿菌PCR和熒光PCR檢測方法的研究
- Windows NT can be used in real time systems. 窗口新臺幣能在真正的時(shí)間系統中被用。
- Establishment and application of quantitative real time PCR assay for detecting duck viral enteritis virus 鴨病毒性腸炎病毒熒光實(shí)時(shí)定量PCR檢測方法的建立和應用
- Fabrication process control in real time. 同步的制造程序控制。
