Methods A549 cells were treated with different cytotoxic drugs for 24hours. The changes of APE/ref-1 expression in A549 after drug-treatment were detected by real time PCR, western blot and streptavidin peroxidase immunohistochemistry staining.
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- 方法 通過(guò)免疫組化染色明確APE/ref-1在A(yíng)549細胞中的表達后,以特定濃度的臨床常用細胞毒性藥物對A549細胞進(jìn)行誘導,采用實(shí)時(shí)熒光定量PCR、Western Blot和免疫組化技術(shù)檢測藥物誘導前后A549細胞內APE/ref-1表達的變化情況。
