Methods Quantitative detection of T cell receptor excision DNA circles (TRECs) in DNA of peripheral blood mononuclear cells from 8 cases with CML, CD4 + or CD8 + cells from 3 cases with CML were preformed by real time PCR (TaqMan) analysis.
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- 方法 利用實(shí)時(shí)定量PCR(Taq Man)方法檢測 8例CML患者外周血單個(gè)核細胞、3例CML患者CD4 + 和CD8+ 細胞中TRECs的水平。