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- ET?1 and NOS mRNA from the gastric mucosa of the three groups were measured quantitatively by Dot blot technique. 采用Dotblot雜交技術定量研究3組胃粘膜ET?1、NOSmRNA表達。
- SCREENING DIFFERENTIALLY EXPRESSED GENES FROM SUPPRESSION SUBTRACTIVE HYBRIDIZATIONAL POSITIVE CLONES USING REVERSE mRNA DOT BLOT TECHNIQUE 利用反向mRNA斑點印跡從抑制消減雜交陽性克隆中篩選差異表達基因
- The 16S rRNA PCR-membrane reverse dot blot hybridization technique showed that the sensitivity was 92.49%, and the specificity was 100%. PCR-膜反向斑點雜交技術鑒定分枝桿菌菌種的靈敏度為92.;49%25;特異度為100%25。
- Effect of labeling was detected by dot blot hybridization. 點雜交方法檢測探針標記效果。
- Southern blot technique developed to identify DNA fragments. 印跡雜交被用來發現DNA片段。
- The purpose of this study was evaluation of RT PCR technique,dot blot hybridization with PCR generated probe and SDS PAGE for the detection of rice black streaked dwarf ?Fijivirus?(RBSDV). 用RT PCR技術、PCR標記的探針點雜交和SDS PAGE檢測了生產上嚴重危害玉米和水稻的水稻黑條矮縮病毒(RBSDV)。
- Methods:Oligonucleotide fragment of Brugia malayi synthesized by genetic engineering technique was labeled with 32P and used as probe.The B.malayi larvae in mosquito was detected with dot blot. 方法:利用基因工程技術合成馬來絲蟲寡核苷酸片段,經32P標記后作為探針,以斑點雜交法檢測蚊體內馬來絲蟲幼蟲。
- Methods Polymerase chain reaction(PCR) connected with reverse dot blot(RDB) were performed. 方法采用多聚酶鏈反應(PCR)結合反向斑點雜交(RDB)技術。
- It was proved that the E2 genes were integrated stably into chromosome of P.Pastoris by Dot blot and DNA sequencing. Pastotis進行整合,經G418篩選得到25個高拷貝轉化子,經DNA斑點試驗和DNA測序證明外源基因E2穩定地整合到P.;Pastoris染色體中。
- Besides, the gene expression of c-myc, wtp53, p16 and EGFR was detected by RNA dot blot. 應用完整細胞原位斑點印跡雜交技術檢測c-myc、野生型p53(wtp53)、p16和EGFR的基因表達;
- Denatured-reduced forms of MBP-fusion proteins in immunoblotting, dot blot and ELISA.General. 特異性 Monoclonal Anti-GFP recognizes wild type; recombinant; and enhanced form of GFP.
- Objective: To study the clinical value of detecting mycobacterium tuberculosis by dot blot hybridization. 摘要目的:探討斑點雜交法檢測結核分枝桿菌的臨床應用價值。
- From recombinant plasmid pGEMTH2 prepared cRNA probe was identified by dot blot hybridization. 使用斑點雜交證實我們制備的探針是敏感而可靠的。
- Rearrangement and amplificarion of erbB,sis,myc and fos in brain tumors are studied with DNA dot blot and Southern blot analysis. 本文用 DNA 斑點雜交法和 Southen 印跡法對腦瘤中 erbB、sis、myc 和 fos 這四種癌基因的擴增和重排進行了研究。
- After random labeling with DIG, RNA dot blot was proceed to test the expression of this gene under different growth condition. 用地高辛隨機引物標記試劑盒對該片段進行標記后,對不同水分和硫營養條件下提取的小麥根系總RNA 進行斑點雜交。
- Dot blot and southern blot analyses suggested that gfp gene is integrated into the genome of transgenic plants of Nicotiana tabacum, Pentunia genome. 通過對有綠色熒光的煙草、矮牽牛進行dot blot、southern blot分析,都獲得陽性雜交結果,證實gfp基因已整合到植物基因組中并與綠色熒光觀察結果一致。
- The supernatant of 20% brain tissue suspension of suckling mice infected with BA66019 strain of XHF virus, were separated by electrophoresis of SDS-PAGE and bloted in NC membranes by western blot technique . 用新疆出血熱病毒原型毒株BA66019感染乳鼠腦的20%25懸液,經差速離心后的上清為抗原進行SDS-PAGE電泳,再用Westernbolt法將凝膠分離的結果印跡到NC膜上。 將6株單克隆抗體小鼠腹水經飽和硫酸銨純化后,在NC膜上進行結合反應。
- Methods:Polymerase chain reaction in combination with dot blot hybridization of allele specific oligonucleotide(PCR/ASO) probes was used. 方法:采用聚合酶鏈反應結合等位基因特異的寡核苷酸探針雜交(PCR/ASO)技術。
- Methods: In patients with AML M2b, karyotype was studied by using G banding technique, AML1/MTG8 fusion gene transcript by using RT PCR and the rearrangement of AML1 gene and MTG8 gene by using Southern Blot technique. 方法 :應用 G顯帶技術分析染色體核型 ,用 RT- PCR法檢測 AML 1/ MTG8融合基因轉錄本并進行微量殘留白血病檢測 ,采用 Southern Blot技術檢測 AML 1及 MTG8基因重排。