The supernatant of 20% brain tissue suspension of suckling mice infected with BA66019 strain of XHF virus, were separated by electrophoresis of SDS-PAGE and bloted in NC membranes by western blot technique .
英
美
- 用新疆出血熱病毒原型毒株BA66019感染乳鼠腦的20%25懸液,經(jīng)差速離心后的上清為抗原進(jìn)行SDS-PAGE電泳,再用Westernbolt法將凝膠分離的結果印跡到NC膜上。 將6株單克隆抗體小鼠腹水經(jīng)飽和硫酸銨純化后,在NC膜上進(jìn)行結合反應。
