Methods:Oligonucleotide fragment of Brugia malayi synthesized by genetic engineering technique was labeled with 32P and used as probe.The B.malayi larvae in mosquito was detected with dot blot.

 
  • 方法:利用基因工程技術(shù)合成馬來(lái)絲蟲(chóng)寡核苷酸片段,經(jīng)32P標記后作為探針,以斑點(diǎn)雜交法檢測蚊體內馬來(lái)絲蟲(chóng)幼蟲(chóng)。
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