It was proved that the E2 genes were integrated stably into chromosome of P.Pastoris by Dot blot and DNA sequencing.

 
  • Pastotis進(jìn)行整合,經(jīng)G418篩選得到25個(gè)高拷貝轉化子,經(jīng)DNA斑點(diǎn)試驗和DNA測序證明外源基因E2穩定地整合到P.;Pastoris染色體中。
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