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- Objective: To assess the change of proadrenomedullin N terminal 20 peptide (PAMP) in patients with essential hypertension. 目的：觀(guān)察原發(fā)性高血壓（EH）與腎實(shí)質(zhì)性高血壓（RH）患者腎上腺髓質(zhì)素前體N端20肽（PAMP）的水平變化，探討PAMP的降壓機制。
- N terminal sequence of both 20 and 5KDa are Met, Pro, Leu, Pro, Pro, His, Pro, Gly, His, Pro. 20KDa、5KDa釉基質(zhì)蛋白N末端前10個(gè)氨基酸殘基均為：Met、Pro、Leu、Pro、Pro、His、Pro、Gly、His、Pro。
- Objective To obtain the recombinant Eotaxin N Terminal Mutant (Met/Eotaxin) advenvirus, and to study its role in allergic diseases. 目的獲取人嗜酸粒細胞親合素(Eotaxin)N端突變體(Met/Eotaxin)重組腺病毒,探討其在小鼠變應性鼻炎中的作用。
- Objective: To obtain the antibody against N terminal of 1A6/DRIM, and thereafter get the profile of 1A6/DRIM expression in different cell lines. 目的 :獲得 1A6 /DRIM的N端特異性抗體并對其在細胞內的定位進(jìn)行分析。
- Objective:To investigate of proadrenomedullin N terminal 20 peptide(PAMP)levels in the plasma of the patients with coronary heart disease(CHD). 目的:觀(guān)察不同類(lèi)型冠心病與腎上腺髓質(zhì)素前體N端20肽(Proadrenomedullin N Terminal 20 Peptide,PAMP)的關(guān)系。
- The first ten of N terminal amino acid sequence of 12 000 protein in GCF was Met, Leu, Thr, Glu, Leu, Glu, Lys, Ala, Leu, Asn. 齦溝液中低相對分子質(zhì)量的 12 0 0 0蛋白N 末端 10個(gè)氨基酸的順序為 :Met、Leu、Thr、Glu、Leu、Glu、Lys、Ala、Leu、Asn。
- Conclusions This result indicate that PCR primer introduced V region N terminal changes may seriously affect the antigen binding activity. 結論在構建小分子抗體時(shí)，PCR引物引入的氨基酸殘基的改變可影響所表達抗體的活性。
- RT PCR showed that all samples expressed HCP gene, only one missense mutation at codon 225 (AAC to AGC, Asn to Ser) within N terminal SH2 domain was found in an ALL patient. RT PCR顯示所有標本中都有HCP基因表達 ,僅在 1例急性淋巴細胞性白血病細胞中發(fā)現一錯義突變 ,發(fā)生在HCP基因氨基末端的SH2結構域 ;
- Objective:To explore the relationship between proadrenomedullin N terminal 20 peptide (PAMP) and left ventricular hypertrophy with essential hypertension(EH). 目的:探討原發(fā)性高血壓(EH)患者血漿中腎上腺髓質(zhì)素前體N端20肽(PAMP)與左室肥厚的相互關(guān)系。
- Their family proteins contain a conserved NAC domain about 150 amino acids in N terminal ends and highly different transcription regulatory domain in C terminal ends. 其家族成員N端含有保守NAC結構域（約150個(gè)氨基酸），C端為高度變異的轉錄激活區。
- The amino acid sequence in all of these 6 clones is the same,HYEFWAWNWEAN, which is hydrophobic as N terminal of C34.Conclusion This phage display peptide can mimic the epitope of HIV 1 gp41 CHR ,and bind with HIV 1 gp41 NHR. 上述 6個(gè)陽(yáng)性克隆經(jīng)DNA測序 ,氨基酸序列相同 :HYEFWAWNWEAN ,其明顯的疏水性質(zhì)類(lèi)似于C34N末端 ,特異性鑒定顯示這些克隆均能夠與HIV 1gp4 1N多肽結合。 結論 該噬菌體克隆展示肽可模擬HIV 1gp4 1CHR多肽表位 ,并可與N多肽結合
- Objective By intravenous administration of P A M P, the N terminal 22 41 ofadrenom edulline precursor,and calcitonin gene related peptide ( C G R P) ,their effect on heart rhyth m in ratwasinvestigated . 目的 為探討腎上腺髓質(zhì)素（ Adreno medullin ， A M） 前體 N 端22?41 的肽（ Pro A M22?41peptide， P A M P） 對心律的影響。
- Keywords ginsenoside;SHSY5Y cells;apoptosis;c jun NH 2 terminal kinase; 關(guān)鍵詞人參皂苷;SHSY5Y細胞;細胞凋亡;絲裂素活化蛋白激酶;
- Objective To elucidate the effect of proadrenomedullin N terminal 20 peptide (PAMP) in cardiovascular system and clinical significance of PAMP plasma concentration in the patients with essential hypertension (EH). 目的 探討腎上腺髓質(zhì)素前體N端 2 0肽 (PAMP)的心血管效應以及原發(fā)性高血壓(EH)患者血漿PAMP的濃度變化。
- Objective: To observe the effect of adrenomedullin (ADM) on the production of nitric oxide (NO) in rat aorta and the effect of Proadrenomedullin N terminal 20 peptide (PAMP) and adrenotensin (ADT) on the ADM induced NO production. 目的 :觀(guān)察腎上腺髓質(zhì)素 (ADM)對大鼠主動(dòng)脈一氧化氮 (NO)生成的作用 ,以及腎上腺髓質(zhì)素原N端 2 0肽 (PAMP)和腎上腺升壓素 (ADT)對ADM這一作用的影響。
- The codons for the 84~(th) and 122~(th) amino acids of hFL were changed to those for E and R, and N terminal codons were changed to the optimal ones for Pichia pastoris to elevate the expression level and to increase the biological activity. hFL第84位和122位氨基酸的密碼子分別改造為E和R的密碼子，并在N端采用甲醇酵母偏愛(ài)密碼子，以提高hFL的活性和表達量。
- TK gene was 534bp and encoded 178 amino acids. On the N terminal of TK, ATP motif was found. TK gene was conserved in CPV, the identity of GTPV-TY TK gene compared with TK gene in other CPV strains were more than 96%. 試驗中首先用PCR方法克隆了GTPV-TY的TK基因,TK基因ORF大小為534bp,編碼178個(gè)氨基酸,TK的N端具有ATP結合結構域,與其它毒株的TK基因的核苷酸序列比較顯示CPV各毒株TK基因的同源性大于96%25,保守性很高。
- AIM To construct the eucaryotic expression vector of the cDNA sequence encoding bioactive N terminal fragment of human bactericidal / permeability increasing protein (BPI) and to express it in COS 7 cells. 目的 構建人殺菌 /通透性增加蛋白 (BPI) N端活性片段 c DNA序列的真核表達載體并了解其在 COS- 7細胞中的表達 .
- AIM: It is to investigate that apoptosis of MDA-MB-435 cell, a human breast cancer cell line, was induced by recombinant retroviral vector mediated CED-4 gene of cadherin 5 N terminal and its apoptosis mechanism. 目的:研究粘附素5 N 端CED-4(cadherin extracellular domain-4)基因經(jīng)重組逆轉錄病毒介導轉染人乳腺癌細胞MDA-MB-435 及其誘導MDA-MB-435 細胞凋亡的分子調控機制。
- Methods: Acetic acid was selected to extract the enamel matrix proteins. The N terminal amino acid sequence of enamel matrix proteins were determined with SDS-PAGE electrophoretic and blot membrnce analysis. 方法：選擇乙酸提取法獲得釉基質(zhì)蛋白，采用SDS－PAGE電泳和印跡膜測序法分析N末端氨基酸序列。
