The codons for the 84~(th) and 122~(th) amino acids of hFL were changed to those for E and R, and N terminal codons were changed to the optimal ones for Pichia pastoris to elevate the expression level and to increase the biological activity.

 
  • hFL第84位和122位氨基酸的密碼子分別改造為E和R的密碼子,并在N端采用甲醇酵母偏愛(ài)密碼子,以提高hFL的活性和表達量。
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