Methods: Acetic acid was selected to extract the enamel matrix proteins. The N terminal amino acid sequence of enamel matrix proteins were determined with SDS-PAGE electrophoretic and blot membrnce analysis.

 
  • 方法:選擇乙酸提取法獲得釉基質(zhì)蛋白,采用SDS-PAGE電泳和印跡膜測序法分析N末端氨基酸序列。
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