您要查找的是不是：
- In addition, a set of expression plasmid vectors, PMS-31b. 同時(shí)構建一組質(zhì)粒表達載體PMS-31b。
- Constructed the expression plasmid pTrc-rCR and checked it. 構建表達質(zhì)粒pTrc-rCR,并酶切檢驗;
- The pPIC9K/Ang-1 yeast expression vector was constructed successfully. 成功構建pPIC9K/Ang-1畢氏酵母表達載體。
- Objective To clone the human angiotensin-converting enzyme 2 (ACE2)and construct its eukaryotic expression plasmid. 目的克隆人血管緊張素轉換酶2基因(ACE2),并構建其真核表達載體。
- Results: Recombinant expression plasmid of ALR was confirmed correct by restriction enzyme digestion and sequencing. 結果：酶切鑒定及測序結果提示表達產(chǎn)物正確；
- Results The bicistronic eukaryotic expression plasmid was corrected and can co - express human BMP -2 and VEGF165 mRNA in vitro. 該重組質(zhì)粒能在體外同時(shí)表達BMP2及VEGF165 mRNA。
- FDR3 was inserted into the yeast expression vector pDBLeu and then was transferred to the yeast strain M3(ctrl mutant) mediated by PEG/LiAc and by electroporation . 把FDR3基因片段克隆到酵母表達載體pDBLeu上，然后通過(guò)電擊和乙酸鋰轉化兩種方法將重組質(zhì)粒導入釀酒酵母Ctrl突變體（M3 mutant）中，采用酵母異源互補法進(jìn)一步鑒定FDR3基因的功能。
- The co expression plasmid encoding FasL and VEGF165 named pCI FasL IRES VEGF165 (simplified as pCI FIV) was successfully constructed. 再以此為基礎構建得到編碼FasL和VEGF165的共表達質(zhì)粒 pCI FasL IRES VEGF165 (簡(jiǎn)稱(chēng)pCI FIV )。
- With high yields of rHSA and relative simple extraction technology,the yeast expression system,especially Pichia pastoris system,is the most prospect system in industrialization. 用酵母表達系統,尤其是畢赤酵母,表達的重組白蛋白產(chǎn)量高且提取工藝簡(jiǎn)單,是其產(chǎn)業(yè)化最具有前途的表達系統。
- Expression and purification of recombinant RTAsThe expression plasmid Pkk223.3-RTA was introduced into E. coli JM 109 by CaCl2-mediated method. 將構建好的重組質(zhì)粒pKK223.;3-RTA和pKK223
- This paper summarized the components of pichia yeast expression system and its main research progresses in the expression of animal infectious pathogen. 總結了畢赤酵母的表達系統的組成及其在表達動(dòng)物傳染病病原方面的主要研究進(jìn)展。
- The experiment results covered several following points:(1) adw was cloned into nuclear plasmid PB, PBG and chloroplast expression plasmid PLCTB . 將乙肝表面抗原基因(adw型)與細胞核載體PB、PBG以及葉綠體表達載體PLCTB進(jìn)行定向克隆，經(jīng)PCR和測序鑒定都獲得了重組子，分別命名為PB-adw、PBG-adw、PLCTB-adw。
- This review focused on progress of Pichia pastoris yeast expression system, the technique of separation and purification of the recombinant proteins recently. 簡(jiǎn)單介紹了目前較為流行的畢赤酵母表達體系，著(zhù)重概述了重組蛋白分離純化技術(shù)方法的應用情況。
- AIM: To construct pET32a/His MBL-CLR recombinant prokaryotic expression plasmid and to express mannan-binding lectin-CLR (MBL-CLR) protein in E. 目的:在大腸桿菌中表達人甘露聚糖結合凝集素(MBL)膠原樣區(CLR)蛋白。
- Methods: 1.The KK gene was directional cloned into the pAAV-MCS. Thisrecombinant pAAV expression plasmid was called pAAV-KK. 方法:1.;將KK 基因定向克隆入腺相關(guān)病毒載體質(zhì)粒pAAV-MCS 中構建成pAAV-KK。
- To construct mammal expression plasmid pcDNA 3.1 ( + )/GDF-5 and check the expression of it in bone marrow mesenchyal stem cells of mice. 目的:通過(guò)基因重組技術(shù)體外構建真核表達質(zhì)粒pcDNA3.;1(+)/GDF-5;并檢測其在小鼠骨髓基質(zhì)干細胞中的表達。
- Methods:The epf gene fragment was amplified by PCR from ZYH24 and cloned into prokaryotic expression plasmid pGEX4T-2 to form pGEX4T-2-epf. 方法根據GenBank S.;suis2epf基因序列設計引物;克隆ZYH24株epf基因片段并進(jìn)行序列分析;
- Then the BLY cDNA fragment was subcloned into prokaryotic expression vector pGEX-4T-1, forming the prokaryotic expression plasmid (pG-BLY). 克隆PCR產(chǎn)物，并構建了pGEX-4T-1-BLY原核表達載體。 經(jīng)BamHI和EcoRI酶切及質(zhì)粒PCR鑒定，證實(shí)本實(shí)驗構建的新型牛溶菌酶基因已克隆到原核表達載體pGEX-4T-1上，為進(jìn)一步研究其誘導表達條件及生物學(xué)功能奠定了基礎。
- The yeast expression vector pREP-COB and the plant expression vector pXL-COB were constructed, and the effection of GhCOBRA overexpression in yeast and cotton is being investigated. 構建了GhCOBRA酵母表達載體pREP-COB和GhCOBRA植物表達載體pXL-COB,準備轉化酵母和棉花來(lái)研究基因過(guò)量表達的功能。
- The customers should provide the gene expression plasmid which has been done the fluorescence labelling. We will provide the detail reports and microscopical photos. 技術(shù)服務(wù)完成后向客戶(hù)提供詳細的實(shí)驗報告和熒光共聚焦顯微鏡照片。
