Methods:The epf gene fragment was amplified by PCR from ZYH24 and cloned into prokaryotic expression plasmid pGEX4T-2 to form pGEX4T-2-epf.

 
  • 方法根據GenBank S.;suis2epf基因序列設計引物;克隆ZYH24株epf基因片段并進(jìn)行序列分析;
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