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- shRNA真核表達載體shRNA eukaryotic expressing vector
- [方法]針對cyclin E基因,設計1對寡核苷酸,形成雙鏈后將其將其插入Psilencer2.1-U6-neo質(zhì)粒,構建cyclin E shRNA真核表達載體,酶切和測序鑒定。[Methods] Oligonucleotides were designed specific for cyclin E gene,after annealing the formed double-stranded DNAs were ligated with Psilencer2.1-U6-neo. The expression vectors of Psilencer2.1-U6/shRNA were identified by enzyme digestion and sequence analysis and transfected into MCF-7 cells.
- 方法構建Survivin基因shRNA真核表達載體pRNAT-U6.1/neo-survivin,轉染人膀胱癌細胞T24并獲得穩定的單克隆細胞。Methods Survivin-targeting shRNA eukaryotic expression vector pRNAT-U6. 1/neo-survivin was constructed and identified. Human bladder cancer cell T24 was transfect-ed with pRNAT-U6. 1/neo-survivin and then stable monoclonal cells were selected.
- 真核表達載體構建Expression vector
- 熒光真核表達載體Fluorescent eukaryotic expressing vector
- 以新霉素抗性基因突變體為篩選標志的真核表達載體的構建Construction of Eukaryofic Expression Vector Using Neomycin-resistance Gene Mutant as Selectable Marker
- HGF非融合蛋白真核表達載體的構建及其在骨骼肌細胞中的表達Construction of HGF Gene Non-fusion Expression Vector and Expression of HGF Protein in Skeletal Muscle Cells
- 真核細胞表達載體eukaryotic expression vector
- 人端粒酶逆轉錄酶基因啟動(dòng)子區的克隆及其真核表達載體的構建Cloning of human telomerase reverse transcriptase gene promoter and construction of its eukaryotic expression vector
- BCR/ABL特異性siRNA真核表達載體構建及其對K562細胞的影響Construction of Eukaryotic Expression Vector of SiRNA Specific for BCR/ABL Fusion Gene and Its Effects on K562 Cells
- 重組真核表達載體Recombinant human eukaryotic vector
- 真核表達載體eukaryotic expression vector
- 促凋亡分子Bad真核表達載體構建及其在人基底細胞癌細胞中的表達Construction of human Bad gene eukaryotic expressing vector and Bad gene expression in human basal cell carcinima cell line
- 真核表達質(zhì)粒載體Eukaryotic expression plasmid
- 真核表達載體pEGFP-C3eukaryotic expression vector pEGFP-C3
- pcDNA3.0-PTEN真核表達載體pcDNA3.0-PTEN vectors
- CEA真核表達載體的瞬時(shí)表達及檢測Transient expression of eukaryotic expression plasmid CEA in vitro and its detection
- APP基因真核表達載體的構建與鑒定The construction of the eukaryotic expression vector of mutation APP gene and its identification
- pEGFP-N1真核表達載體Eukaryotic expression vector pEGFP-N1
- 真核表達載體的構建及表達Construction and expression of human anti-HAV Fab eukaryotic expression vector
