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- 真核表達載體pEGFP-C3eukaryotic expression vector pEGFP-C3
- 成功構建hKu70基因反義RNA真核表達載體pEGFP C1 K ,為建立該基因低表達細胞株、DNA雙鏈斷裂修復缺陷和有關(guān)毒理學(xué)研究提供工具The efficient expression vector of hKu 70 gene antisense RNA,pEGFP|C1|K has been constructed successfully,it can be used to establish the human cell line which express lower hKu 70 protein and in researches about DSB repair deficiency and toxicology.
- 方法:采用基因克隆技術(shù),將合成的短發(fā)卡樣特異性PTI1(PC3)RNA干擾寡核苷酸序列插入真核表達載體pEGFP/U6,構建PTI1(PC3)shRNA的真核表達載體,體外轉染人前列腺癌PC3細胞,48h后觀(guān)察細胞生物學(xué)變化;METHODS: The shRNA specific to PTI-1 (PC-3) was synthesized and inserted into pEGFP/U6 by gene recombination technology to construct the expression vector, pEGFP/U6-mPs, which was then transfected into the cultured PC-3 cells.
- 目的 構建人DNA雙鏈斷裂 (DSB)修復基因hKu70反義RNA真核表達載體pEGFP C1 K ,為以后的hKu70基因功能和毒理學(xué)研究提供實(shí)驗材料。Objective To construct the pEGFP|C1|K vector,an eukaryotic expression plasmid of DNA double|strand break(DSB) repair gene hKu 70 antisense RNA and provide experimental material for the studies of hKu 70 gene function and toxicology.
- pEGFP-N1真核表達載體Eukaryotic expression vector pEGFP-N1
- 經(jīng)測序證實(shí),GCRG213正確克隆入真核表達載體pEGFP-Nl,組成陽(yáng)性重組子pEGFP-Nl-GCRG213。 測序正確的pEGFP-N1-GCRG213重組子經(jīng)脂質(zhì)體轉染COS-7細胞,激光共聚焦顯微鏡觀(guān)察GCRG213蛋白在胞核和胞質(zhì)中均有表達。DNA sequence analysis showed that the GCRG213 sequence was right in the recombinant plasmid pEGFP-N1-GCRG213, transiently transfecting the eukaryotic cell COS-7 with pEGFP-N1-GCRG213. By using the confocal technique, GCRG213 was identified to be expressed in the cytoplasm and nuclei of COS-7 cells.
- 以新霉素抗性基因突變體為篩選標志的真核表達載體的構建Construction of Eukaryofic Expression Vector Using Neomycin-resistance Gene Mutant as Selectable Marker
- HGF非融合蛋白真核表達載體的構建及其在骨骼肌細胞中的表達Construction of HGF Gene Non-fusion Expression Vector and Expression of HGF Protein in Skeletal Muscle Cells
- 人端粒酶逆轉錄酶基因啟動(dòng)子區的克隆及其真核表達載體的構建Cloning of human telomerase reverse transcriptase gene promoter and construction of its eukaryotic expression vector
- BCR/ABL特異性siRNA真核表達載體構建及其對K562細胞的影響Construction of Eukaryotic Expression Vector of SiRNA Specific for BCR/ABL Fusion Gene and Its Effects on K562 Cells
- 真核表達載體構建Expression vector
- 真核表達載體eukaryotic expression vector
- 促凋亡分子Bad真核表達載體構建及其在人基底細胞癌細胞中的表達Construction of human Bad gene eukaryotic expressing vector and Bad gene expression in human basal cell carcinima cell line
- 熒光真核表達載體Fluorescent eukaryotic expressing vector
- 真核表達質(zhì)粒載體Eukaryotic expression plasmid
- pcDNA3.0-PTEN真核表達載體pcDNA3.0-PTEN vectors
- shRNA真核表達載體shRNA eukaryotic expressing vector
- 重組真核表達載體Recombinant human eukaryotic vector
- 以L(fǎng)ipofectamineTM2000試劑轉染pEGFP-N3-GAP-43表達載體至COS-7細胞中進(jìn)行瞬時(shí)真核表達。The GAP-43 gene was inserted into eukaryotic expression vector pEGFP-N3.The recombinant expression vector was transiently transfected into COS-7 cells by LipofectamineTM 2000 reagent.
- 真核表達載體的構建及表達Construction and expression of human anti-HAV Fab eukaryotic expression vector
