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- Methods The full length of CD95 cDNA was inserted into the multiple cloning site of the expression vector pBK CMB by molecular cloning technique. 方法 采用分子克隆技術(shù)將CD95基因插入真核表達載體pBK CMV的多克隆克隆位點(diǎn)之間 ,以脂質(zhì)體介導法將目的基因導入受體細胞SGC790 1,用G418篩選克隆細胞 ;
- The first item: monoclone antibody and multiple clone antibody have exerted abroad in biology research, clinic diagnosisand therapy field.It"s a huge market. 其中即將上馬的第一個(gè)項目:?jiǎn)慰寺】贵w和多克隆抗體在生物學(xué)研究、臨床診斷和治療等領(lǐng)域中已被廣泛運用,具有非常廣闊的市場(chǎng)前景。
- In the Tool:Topology subpalette, select the cloned model only fingers of which are visible. 模式,這樣新生成的模型將投射到舊的模型模板上。
- While most of Baker's clone characters are recorded separately, he sometimes voices multiple clones at once, depending on the complexity of the dialogue and the number of clones in the scene. 雖然絕大多數貝克的克隆人角色都是分別錄音的,但他有時(shí)也要根據對話(huà)的復雜程度以及場(chǎng)景中的克隆人數,一次配多個(gè)克隆人。
- The products of PCR werenamed Sa and Sb, of 2.1Kb and 2.3Kb respectively. Sa was inserted in EcoR I andKpn I sites after Sb was cloned in KpnI and PstI multiple cloning sites of the samepUC18 plasmid. 1Kb和2.;3Kb兩個(gè)片段,經(jīng)酶切鑒定后,先后插入到pUC18質(zhì)粒載體上的EcoRI和PstI多克隆位點(diǎn)上,構建了重組質(zhì)粒pUC-S。
- METHODS: The HAb18GEF gene fragment was amplified by PCR and digested with Sac I/Not I,and then cloned into the multiple clone site downstream of EpoR/LR-F3 gene of eukaryotic expression vector pCEL2f. 方法:用PCR法擴增肝癌相關(guān)抗原HAb18G胞外段基因,SacI/NotI雙酶切后插入真核表達載體pCEL2f中,構建EpoR/LR-F3/HAb18GEF嵌合受體基因的重組表達載體pCEL2f/HAb18GEF,并經(jīng)酶切和測序驗證。
- The gene segments mentioned above were cloned into the multiple cloning sites of retrovirus vector pLXSN to construct an attenuated superantigen expression vector pLXSN SEA (D227A) linker CD80tm modulated by cis acting element of AFP gene. 將上述片斷插入逆轉錄病毒載體 pLXSN的多克隆位點(diǎn) ,構建AFP基因順式作用元件調控的肝癌特異性減毒超抗原表達載體 (pLXSNSEA(D2 2 7A) linker CD80tm)。
- He is the person with multiple injuries. 他就是那個(gè)多處受傷的人。
- Friendship multiple joy and divide grieve. 朋友之間,歡樂(lè )同享,苦難同當。
- We use the hall for multiple purposes. 這個(gè)大廳我們派多種用途。
- Expression and purification of enhanced green fluorescent protein and preparation of multiple cloning antibody 增強型綠色熒光蛋白原核表達純化與多克隆抗體的制備
- A multiple crash can often be seen on that motorway. 那條公路上的聯(lián)環(huán)撞車(chē)事故很多。
- So who is most likely to want or use human cloning? 所以誰(shuí)最有可能需要或使用人類(lèi)克隆技術(shù)呢?
- Cloning differs from genetic engineering. 克隆與基因工程不同。
- These are inserted into a cloning vector. 它們被插入克隆載體。
- The actual process of cloning is very technical. 克隆的實(shí)際過(guò)程有很強的技術(shù)性。
- The despot will not to the cloning lab today. 暴君今天不去克隆實(shí)驗室。
- He then proposed a five-year ban on cloning. 后來(lái)他又提出對克隆技術(shù)的五年禁期。
- The U.S. Congress should ban human cloning now. 美國國會(huì )應該現在就禁止人的克
- Will the human cloning really be born? 克隆人真的會(huì )誕生嗎?