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- The Tat signal peptide detecting vector of PGA were constructed. 構建了Tat信號肽檢測載體。
- We add the 5'AOX1 primer and 3'AOX1 primer amplify pPIC9K and alpha-factor signal peptide sequence was obtained. 考慮到克隆方便,在序列5’端和3’端分別引入BamHI和EcoRI 位點(diǎn),并在兩個(gè)酶切位點(diǎn)的外側分別加上保護堿基。
- Once across the membrane, the signal peptide is cleaved by the signal peptidase and the process protein is released. 信號肽是疏水的，與膜上結合的蛋白接近，拉著(zhù)后面的親水多肽跨膜，一旦跨過(guò)膜，即被信號肽酶切除，釋放出加工的蛋白。
- The location of the signal peptide of this peptide chain is 1-26, the breaking point is between 26 and 27 (GAA-AF). 應用神經(jīng)網(wǎng)絡(luò )算法和隱馬氏模型分析結果顯示該肽鏈信號肽位置為1-26，斷裂點(diǎn)位置是26-27（GAA-AF）。
- The extramembrane region consists of 758 aminoacids, including signal peptide containing 31 amino acids at N-terminus and seven Ig-like domains. 胞外區由758個(gè)氨基酸組成，包括N端31個(gè)氨基酸的信號肽和七個(gè)類(lèi)似免疫球蛋白(Ig)的結構域。
- There were no signal peptide and N-glycosylation sites, while had 4 conservative Cys amino acid in the deduced amino acid sequence. 在推導的貓IL-18氨基酸序列中,無(wú)信號肽序列和潛在的N-聯(lián)糖基化位點(diǎn),但存在4個(gè)Cys殘基。
- The signal peptide at the N- termination can lead the protein to the periplasm.Six histidines at the C- termination will let the purification easy. 在質(zhì)粒pET-20b(+)中，重組的蛋白會(huì )在N-末端帶有一個(gè)信號肽序列，它可以使重組蛋白分泌到細胞的周質(zhì)空間處，而其在C-末端還有帶有6個(gè)組氨酸的標簽，這樣就使得重組蛋白的分離純化更加的方便，簡(jiǎn)單。
- Proteins secreted by one step into the periplasm or through Bayer's junctions to the extracellular environment have a signal peptide. 蛋白可直接分泌到周質(zhì)中，或通過(guò)Bayer接點(diǎn)分泌到胞外環(huán)境中的蛋白有一個(gè)信號肽。
- In this paper, signal peptide was added before the N-terminus of CEMA with the intention to reduce its toxic to plant cells while maintaining the strong antibacterial activity. 本文通過(guò)在CEMA的N端添加信號肽，對其進(jìn)行改造，以探索不降低抑菌作用的同時(shí)，降低其對植物細胞毒害作用的可能性。
- GliTd-3 and GliTd-4 were both 861bp, and their deduced amino acid sequences were both 267 residues with a 19-residue putative signal peptide (absent from mature proteins). 而GliTd-1和GliTd-2編碼區分別長(cháng)821bp和859bp，由于編碼區內存在提前終止密碼子和插入/缺失引起的移碼突變，推測其為不表達的假基因。
- With the signal peptide analysis software of the TMHMM2.0(http://genome.cbs.dtu.dk/services /TMHMM-2.0), the preceding peptide of Vip3A-WB5 was a transmembrane peptide. 通過(guò)TMHMM2.;0(http://genome
- Fibroblast growth factor（FGF-1）lacks amino-terminal signal peptide, so it can’t release through the classical endoplasmic reticulum（ER）-Golgi pathway. 酸性成纖維生長(cháng)因子-1（FGF-1）由于氨基端缺乏信號肽序列，不能通過(guò)經(jīng)典的內質(zhì)網(wǎng)-高爾基體途徑釋放。
- With common molecular biology technique,we cloned SCF gene including signal peptide coding sequence coded signal peptide and constructed its expression plasmid. 采用常規的分子生物學(xué)技術(shù),從小鼠骨髓細胞中克隆了含信號肽序列的SCF,并構建了表達載體。
- This article reviews signal peptide sequence about its structural feature,the common used signal peptide and the construction of vector with high secretory. 本文將從信號肽結構特征、常用的信號肽序列以及構建高分泌型載體等方面進(jìn)行綜述。
- Signal peptide was found on former 20 amino acids of both two coding regions of protein, one strong and one feeble transmembrane helices were also found. 2. 并在兩種酶蛋白編碼區的前20個(gè)氨基酸發(fā)現信號肽序列，通過(guò)跨膜區預測，均發(fā)現一個(gè)較強的跨膜信號和一個(gè)較弱的跨膜信號。
- The fusion DNA fragment of the lactococcal extracellular protease USP45 signal peptide, passenger protein and SBC were expressed in Lactococcus lactis and Lactobacillus paracasei. 融合乳酸菌胞外蛋白酶USP45訊息勝肽、乘載蛋白及表層蛋白質(zhì)靜默基因C端123個(gè)胺基酸，表現于副乾酪乳桿菌或乳酸鏈球菌。
- Obtaining of SPCEMA(signal peptide modified CEMA)SPCEMA(187bp) was amplified with two long complementary primers (P2 and P3) and two primers (Pl and P4) containing restriction enzyme recognition site. 以?xún)蓷l部分重疊長(cháng)鏈引物P_2和P_3延伸產(chǎn)物為模板，P_1和P_4為正、反引物進(jìn)行PCR擴增，獲得了改造的抗菌肽基因SPCEMA(187bp)。
- The signal peptide, KDEL and E tag sequence was successfully introduced into 9.1C3 ScFv, and the intrabody gene was inserted into eukaryotic expression vector which can be expressed in COS7 cells. DNA序列測定證明 ,通過(guò)PCR成功地為 9 1C3ScFv引入了內質(zhì)網(wǎng)定位所需的信號肽、KDEL及E tag序列 ,成功地構建了胞內抗體真核表達載體 ,通過(guò)胞內染色方法和Westernblot證明胞內抗體在COS7中得到表達。
- Sequence analysis of Gal-4 eDNA showed that the cDNA of Gal-4 gene con- sists of 321 bp,encoding Gal 4 polypeptide of 63 amino acids,which comprised of signal peptide with 20 amino acids at N'terminus,and mature peptide with 38 amino acids. 序列分析結果表明,獲得的廣西黃雞Gal-4基因大小為321 bp,推導的Gal-4由63個(gè)氨基酸組成,其中N端20個(gè)氨基酸為信號肽,C端38個(gè)氨基酸組成Gal-4的成熟肽。
- The SJCWL06 gene is 861 bp in whole length, has an open reading frame (ORF) of 327 bp encoding 109 amino acids with a signal peptide, an ABC-ATPase domain, several phosphorylation sites and the highest similarity of SMC3 protein of Apis mellifera (84%). 該基因長(cháng)861bp,有一個(gè)完整的327bp的開(kāi)放讀框,編碼109個(gè)氨基酸,其編碼蛋白有一個(gè)信號肽,多個(gè)磷酸化位點(diǎn)和一個(gè)ABC-三磷酸腺苷酶的結構域,與蜜蜂(Apis mel-lifera)SMC3編碼的mRNA基因序列同源性最高(84%25)。
