Obtaining of SPCEMA(signal peptide modified CEMA)SPCEMA(187bp) was amplified with two long complementary primers (P2 and P3) and two primers (Pl and P4) containing restriction enzyme recognition site.
英
美
- 以?xún)蓷l部分重疊長(cháng)鏈引物P_2和P_3延伸產(chǎn)物為模板，P_1和P_4為正、反引物進(jìn)行PCR擴增，獲得了改造的抗菌肽基因SPCEMA(187bp)。
