The signal peptide, KDEL and E tag sequence was successfully introduced into 9.1C3 ScFv, and the intrabody gene was inserted into eukaryotic expression vector which can be expressed in COS7 cells.
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- DNA序列測定證明 ,通過(guò)PCR成功地為 9 1C3ScFv引入了內質(zhì)網(wǎng)定位所需的信號肽、KDEL及E tag序列 ,成功地構建了胞內抗體真核表達載體 ,通過(guò)胞內染色方法和Westernblot證明胞內抗體在COS7中得到表達。
