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- Hygromycin B resistance was studied on the mycelium and protoplast of Lentinula edodes and Pleurotus ostreatus. 本實(shí)驗首先確定潮霉素濃度對香菇和平菇菌絲和原生質(zhì)體再生的影響。
- T he concentration and method of hygromycin selection are determined through contr ast experiment. 通過(guò)比較試驗，確定了潮霉素篩選的濃度和方法。
- The hygromycin is suitable to selection of castor transfomants while castor embryo axis is insensitive to kanamycin. 蓖麻胚軸對卡那霉素不敏感，潮霉素是蓖麻轉化的適宜篩選劑。
- The regenerated fertile plants were obtained by effective selection for hygromycin B resistance. ）幼胚誘導的愈傷組織，經(jīng)潮霉素抗性篩選，得到可育的再生植株。
- Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis by disrupting translocation and promoting mistranslation at the 80S ribosome. 潮霉素B是一種氨基糖甙抗生素，它通過(guò)干擾轉位和促進(jìn)80S核糖體上的錯譯從而抑制蛋白質(zhì)的合成。
- Results showed that Micromonospora sp. 40027 was sensitive to thiostrepton,apramycim,streptomycin and capreomycin,and resistant to kanamycin, hygromycin and gentamycin. 而對卡那霉素、潮霉素和慶大霉素表現抗性。
- Utilizing this system, more than 50% hygromycin resistance selection efficiency was obtained with callus derived from young embryo (DREW, japonica ) and young panicle (D Shan B, indica ) as receptor, some regenerated plants were acquired. 依據該體系,以粳秈DREW 的幼愈傷組織和秈稻D汕B的幼穗愈傷組織為受體,經(jīng)農桿菌AGL1/pCAMBIA1301轉化后, 獲得了超過(guò)50%25 的潮霉素抗性愈傷篩選率, 并獲得再生植株。
- This effect makes it possible to eliminate all tumor cells despite only a fraction of them expressing HSV1 -tk gene. In this study, A fusion gene encoding both HSV1-TK and hygromycin phosphotransferase was used as the therapeutic gene. 本研究用一種與潮霉素磷酸轉移酶基因(hygromycinphosphotransferase gene，hph)相融合的HSV1-tk基因作為目的基因(稱(chēng)為HyTK基因)，構建了重組逆轉錄病毒載體LNCHyTK；
- By the means of leave assay of hygromycin resistance, PCR amplification of a HPT fragment and Southern blot, it showed that RCOI and OsAOS fragments were successfully inserted into the rice genome. 利用離體葉片潮霉素快速鑒定、潮霉素PCR和Southern雜交等方法對轉基因植株后代進(jìn)行鑒定，證明了RCOI和OsAOS基因的片段正確插入水稻基因組；
- Fig.1 The Construction of plant transformation vector of anti-sense gene of RbThe transcript director of Rb is opposite to 35S promoter.Thisvector has GUS report gene and hygromycin resistance gene. 標題： 圖1 Rb反義基因表達載體結構示意圖此表達載體Rb的轉錄方向與35S啟動(dòng)子的轉錄方向相反,并且含有GUS報道基因和抗潮霉素基因。
- PCR analysis and histochemical assay of GUS activity are conducted to testify the transformation events and the successful expression of reporter gene in independent hygromycin resistant calli. 為建立銀杏遺傳轉化的高效體系，對一些重要的影響銀杏轉化的因素，包括外植體類(lèi)型，選擇標記和篩選壓，農桿菌工程菌株，共培養時(shí)間和恢復時(shí)間第一次進(jìn)行了試驗并優(yōu)化了條件。
- The time for hygromycin selecting 篩選時(shí)間
- hygromycin B phosphotransferase gene 潮霉素磷酸轉移酶
- Hygromycin B phosphofransferose gene 潮霉素B磷酸轉移酶基因
- Study on selection efficiency of rice calli with hygromycin and PPT 潮霉素和PPT對水稻愈傷組織篩選效果的比較
- Stability of hygromycin resistant transformant of Colletotrichum lagenarium A, 潮霉素抗性轉化子的穩定性
- Keywords Aspergillus fumigatus;Agrobacterium tumefaciens;Hygromycin resistance; 煙曲霉;根癌農桿菌;潮霉素抗性;
- Keywords Hygromycin B phosphotransferase;Monoclonal antibody;Identification; 單克隆抗體;鑒定;
- Keywords Hygromycin;Resistance;Feeder;BDF1 mice;transgenic;Model;animal; 潮霉素;飼養層;小鼠;BDF1;轉基因;模型;動(dòng)物;
- homomycin 勻霉素
