PCR analysis and histochemical assay of GUS activity are conducted to testify the transformation events and the successful expression of reporter gene in independent hygromycin resistant calli.
英
美
- 為建立銀杏遺傳轉化的高效體系,對一些重要的影響銀杏轉化的因素,包括外植體類(lèi)型,選擇標記和篩選壓,農桿菌工程菌株,共培養時(shí)間和恢復時(shí)間第一次進(jìn)行了試驗并優(yōu)化了條件。