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- Results In PCR, the primer for internal reference gene, content of dNTP, and runing cycles affect the products much than the annealing temperature. 結果在pcr反應中,擴增內參照和目的基因的引物比例、底物濃度以及pcr反應的循環(huán)次數對產(chǎn)物的影響較大,而退火溫度的影響較小。
- We optimized the concentration of Mg2+ ,dNTP and primers in PCR reaction mixture by amplifying the RNAtranscribed in vitro above. 對 RT-PCR 反應體系中的 Mg2+,dNTP 和引物濃度進(jìn)行優(yōu)化。
- This suggests that harringtonine might block the polymerization of dNTP into DNA, but without influence on the phosphorylation of TdR. 說(shuō)明三尖杉酯堿不抑制胸腺嘧啶核苷的磷酸化,而抑制脫氧核苷三磷酸到DNA的聚合過(guò)程。
- Method of DNA extraction and factors influencing RAPD analysis,including the concentration of DNA template, Mg 2+ , dNTP, primers and Taq, in jute were studied. 以假黃麻、假長(cháng)果、越南圓果 (圓果種黃麻 )等為材料 ;研究了黃麻 DNA的提取方法以及對 RAPD分析的影響因素 ;包括模板濃度、Mg2 + 、d NTP、引物和 Taq酶等 ;建立了適于黃麻種質(zhì) RAPD分析的 PCR反應體系 .
- Factors which affect the ISSR analysis of Abies beshanzuensis,such as annealing temperature and concentrations of template DNA,Taq DNA polymerase,Mg~2+ and dNTP,etc. were examined. 以百山祖冷杉DNA為材料;分析了DNA濃度、Mg2+濃度、dNTP濃度、Taq酶的含量以及退火溫度對ISSRPCR擴增結果的影響;經(jīng)過(guò)優(yōu)化實(shí)驗建立了百山祖冷杉ISSRPCR最佳反應體系.
- The ladder experiments showed: the best PCR amplified system of the crested ibis is as follows: Primer(10pmol/ul)lul, Taq enzyme(lU/ul)1.5ul, dNTP(2.5mM)2.0ul, Mg2+(25mM)2.5ul, teplate DNAlOng, TH2O17ul, and final volume is 25ul. 本試驗對各種影響因素進(jìn)行了梯度篩選，得到朱?最佳的PCR擴增體系為：引物(10pmol/ul)1ul、TaqDNA聚合酶(1U/ul)1.;5ul、dNTP(2
- deoxyribonucleoside triphosphate(dNTP) 脫氧核糖核苷三磷酸
- dNTP concentration grads dNTP濃度梯度
- dinitrothiophosphate 二硝基硫代磷酸鹽(酯)
- dNTP (=deoxy-ribonucleoside triphosphate) 三磷酸脫氧核(糖核)苷
- DNTP(parathion) 對硫磷[殺蟲(chóng)藥]
- cDNA was synthesized by reverse transcription (RT). The total volume of reaction system was 20ulincluded 4uL MgCL2, 2ul IQxRNAPCRbuffer, 2ul dNTP mixture, O. Sul Rnase Inhibitor, lul Random 9mers, lul Reverse Transcriptase, lug sample. 逆轉錄(RT)合成cDNA，反應體系20UL包括：MgCL_24uL，10×RNA PCR緩沖液2ul，dNTP混合液2ul，RNA酶抑制物0.;5ul，隨機引物1ul，逆轉錄酶1ul，樣品RNA 1ug。
