cDNA was synthesized by reverse transcription (RT). The total volume of reaction system was 20ulincluded 4uL MgCL2, 2ul IQxRNAPCRbuffer, 2ul dNTP mixture, O. Sul Rnase Inhibitor, lul Random 9mers, lul Reverse Transcriptase, lug sample.
英
美
- 逆轉錄(RT)合成cDNA，反應體系20UL包括：MgCL_24uL，10×RNA PCR緩沖液2ul，dNTP混合液2ul，RNA酶抑制物0.；5ul，隨機引物1ul，逆轉錄酶1ul，樣品RNA 1ug。
