The ladder experiments showed: the best PCR amplified system of the crested ibis is as follows: Primer(10pmol/ul)lul, Taq enzyme(lU/ul)1.5ul, dNTP(2.5mM)2.0ul, Mg2+(25mM)2.5ul, teplate DNAlOng, TH2O17ul, and final volume is 25ul.

 
  • 本試驗對各種影響因素進(jìn)行了梯度篩選,得到朱?最佳的PCR擴增體系為:引物(10pmol/ul)1ul、TaqDNA聚合酶(1U/ul)1.;5ul、dNTP(2
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