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- The investigator needs only to supply the cloned DNA containing a good E. coli promoter and ribosome binding sites. 研究者只需準備含有合適的原核啟動(dòng)子和核糖體結合位點(diǎn)的克隆DNA就行。
- Protein Expression: A Practical Approach details the expression of cloned DNA or RNA templates in all the major in vivo and in vitro systems. 蛋白質(zhì)表示: 一實(shí)際方法細節克隆DNA 或者RNA 模板在全部主要方面的表示在活體內和在試管內系統。
- In this system, cloned maize mitochondrial DNA(mtDNA) is served as template. this is the first established cell-free system for in vitro transcription of cloned DNA with a plant RNA polymerase B. 這是首次用克隆化DNA為模板，以植物RNA聚合酶B建立起的無(wú)細胞離體轉錄系統。
- B7 1(CD80) cDNA was cloned by RT PCR from human B lymphma Raji cell line and confirmed by DNA sequencing. 應用RT－PCR技術(shù)從人B淋巴瘤細胞系Raji中克隆到B7－1（CD80）cDNA，并經(jīng)測序證實(shí)。
- Complementary DNA (cDNA) sequence of Japanese sea bass (Lateolabrax japonicus) prion protein (PrP) encoding gene was cloned and characterized through RT-PCR approach. 采用RT-PCR方法分離了鱸魚(yú)蛋白質(zhì)感染因子蛋白編碼基因序列,并進(jìn)行了結構分析。
- METHODS: Based on the cDNA sequence of DCN reported by GenBank, DCN segment with open reading frame was cloned into plasmid UF1 (UF1-DCN) after DNA sequencing. 方法:根據GenBank報道的DCN基因cDNA序列,克隆含開(kāi)放讀碼框的DCN基因片段,測序后克隆至質(zhì)粒UF1(UF1·DCN),通過(guò)磷酸鈣鄄DNA沉淀法共轉染293細胞,產(chǎn)生高滴度的含DCN基因的重組腺相關(guān)病毒(rAAV·DCN)。
- The ORF of two circadian gene BMAL1 and CRY1 were cloned from normal human whole brain marathon cDNA and verified with restriction enzyme digestion and DNA sequencing. 從正常人全腦marathon cDNA中克隆到人生物節律基因BMAL1和CRY1的開(kāi)放讀碼框架(ORF)，經(jīng)酶切和測序鑒定與GeneBank數據庫中收錄的序列基本一致。
- Each clone would have the same DNA. 每一個(gè)克隆人都有著(zhù)同樣的dna。
- A new gene APG was cloned and its cDNA full-length is 247bp. 克隆了一個(gè)肝癌凋亡相關(guān)基因,cDNA全長(cháng)為563bp。
- Fast Method for Cloning DNA Big Fragments 快速克隆DNA大片段的方法
- Objective: To clone and sequence cDNA of human lymphotoxin (LT). 目的：克隆人淋巴毒素cDNA并測序。
- Characterization of A Cloned DNA Fragment of Rice Chloroplast and Location rbcL Gene in the Recombinant Plasmid 水稻葉綠體DNA克隆片段的分析和rbcL基因在重組質(zhì)粒上的定位
- 3、For genomic analysis, one does not typically sequence dictly from clone DNA carried in a lambda vector. why not? 酵母人工染色體的控制系統具有三個(gè)部分：著(zhù)絲粒、端粒、酵母自主復制序列。還有選擇標記基因。
- CHS gene was cloned from cDNA and CHS open reading frame sequence was obtained. 獲得了CHS基因ORF(Open reading frame)的cDNA序列，全長(cháng)為1299bp。
- Each plasmid contains a cDNA insert that was cloned via EcoRI and XhoI. 每個(gè)質(zhì)體含有一段cDNA，此插入基因是利用EcoRI與XhoI來(lái)復殖的。
- We have cloned a cDNA sequence of the human kinase SBK1(homo sapiens SH3-binding domain kinase 1)for the first time. The genomic DNA sequence of human SBK1 has been acquired through an electronic clone by the bioinformatics tools. 首次克隆到人的SBK1(homo sapiens SH3-binding domain kinase1;SBK1)的cDNA序列;并通過(guò)生物信息學(xué)的手段;電子克隆到人SBK1的基因組DNA序列.
- DNA fingerprinting can help the police to find the criminals. DNA指紋術(shù)有助於警察發(fā)現罪犯。
- METHOD:PF4 complementary deoxyribonucleic acid (cDNA) was amplified by polymer ase chain reaction (PCR), then cloned into pUC19 vector. After sequencing,PF4-c DNA was subcloned into eukaryotic expression vector pIRES2-EGFP. 方法:人工合成PF4基因模板,通過(guò)PCR方法擴增PF4-cDNA,然后克隆至pUC19克隆載體,經(jīng)序列測定后重組入pIRES2-EGFP真核表達載體并進(jìn)行酶切鑒定。
- Objective:To clone HLA A *0201 cDNA and express it transiently on COS 7 cells. 目的 :克隆HLA A 0 2 0 1編碼區的cDNA ,并建立在COS 7細胞上的瞬時(shí)表達系統。
- Expression of the Tea cystatin cDNA in E. coli[J]. 引用該論文 王朝霞;江昌俊;余有本;房婉平.
