METHOD:PF4 complementary deoxyribonucleic acid (cDNA) was amplified by polymer ase chain reaction (PCR), then cloned into pUC19 vector. After sequencing,PF4-c DNA was subcloned into eukaryotic expression vector pIRES2-EGFP.
英
美
- 方法:人工合成PF4基因模板,通過(guò)PCR方法擴增PF4-cDNA,然后克隆至pUC19克隆載體,經(jīng)序列測定后重組入pIRES2-EGFP真核表達載體并進(jìn)行酶切鑒定。
