METHODS: Based on the cDNA sequence of DCN reported by GenBank, DCN segment with open reading frame was cloned into plasmid UF1 (UF1-DCN) after DNA sequencing.
英
美
- 方法:根據GenBank報道的DCN基因cDNA序列,克隆含開(kāi)放讀碼框的DCN基因片段,測序后克隆至質(zhì)粒UF1(UF1·DCN),通過(guò)磷酸鈣鄄DNA沉淀法共轉染293細胞,產(chǎn)生高滴度的含DCN基因的重組腺相關(guān)病毒(rAAV·DCN)。
