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- Conclusion The SNP fluorescent-multiplex system based on the fragment length discrepant allele specific PCR strategy is simple and economical, and is of a high application value in forensic medicine. 結論采用熒光標記復合擴增毛細管電泳法進(jìn)行SNP分型，方法簡(jiǎn)單實(shí)用，在法醫學(xué)個(gè)人識別領(lǐng)域具有較高的應用。
- We genotyped some main Chinese sweet cherry cultivars for the first time using the alleles specific PCR, and two new S genotype (S1S6, S4S9) were found, which made the listing of S-allele assignment more completed. 對57個(gè)國內外的甜櫻桃品種進(jìn)行了自交不親和(SI)基因型的鑒定，首次鑒定了我國主栽的一些甜櫻桃品種的S基因型，并發(fā)現了兩個(gè)新的S基因型S1S6、S4S9，將自交不親和的組群擴展到21個(gè)。
- Keywords Plasmodium vivax;circumsporozoite protein（CSP）;nest allele specific PCR;different size DNA band; 間日瘧原蟲(chóng);環(huán)子孢子蛋白基因;套式等位特異PCR;多樣性DNA片段;
- Keywords Forensic biological evidence;Y chromosome;Biallelic marker;Tragment length discrepant allele specific PCR;Haplogroup; 法醫物證學(xué);Y染色體;雙等位基因標記;片段長(cháng)度差異等位基因特異性PCR;單體群;
- allele specific PCR 等位基因特異性多聚酶鏈反應
- allele specific PCR(AS-PCR) 等位特異性PCR, 等位基因特異性擴增技術(shù)
- Methods:Polymerase chain reaction in combination with dot blot hybridization of allele specific oligonucleotide(PCR/ASO) probes was used. 方法：采用聚合酶鏈反應結合等位基因特異的寡核苷酸探針雜交（PCR/ASO）技術(shù)。
- The methylation specific PCR (MSP) and bisulfite DNA sequencing were performed to examine the methylation status of SLIT2 gene promoter. 亞硫酸氫鈉處理DNA,用MSP和測序法檢測SLIT2啟動(dòng)子甲基化狀態(tài)。
- The sensitive and specific PCR method for detection E. granulosus from dog faeces was preliminarily developed. 初步建立了靈敏、特異的檢測家犬糞便中細粒棘球絳蟲(chóng)的PCR方法。
- Method Several pairs of specific PCR primers were designe d according to the conserved region of HDV genome. 方法針對HDV基因保守區域設計多對PCR引物。用芯片點(diǎn)樣儀將PCR產(chǎn)物點(diǎn)到玻片上制成基因芯片。
- Conclusion The sensitive and specific PCR method for detection E. granulosus from dog faeces was preliminarily developed. 結論初步建立了靈敏、特異的檢測家犬糞便中細粒棘球絳蟲(chóng)的PCR方法。
- Methods The methylation status of CpG island of AR gene promoter in 3 leukemic cell lines and 15 leukemic bone marrow cells was detected by methylation specific PCR(MSP). 方法應用甲基化特異性PCR技術(shù)(methylation specific PCR,MSP)檢測3種白血病細胞株及15例患者骨髓標本雄激素受體基因甲基化狀態(tài)。
- Methods The specific PCR primers were designed and synthesized to amplify the DNA fragments enconding preS1/S2/S antigen gene from the plasmid pHBV adr by PCR method. 方法設計合成2對寡核苷酸引物,以adr亞型HBV質(zhì)粒pHBV DNA為模板,采用PCR法分別擴增HBV大分子表面蛋白基因(preS1/S2/S基因)片段;
- The established specific PCR assay should provide a practical and rapid method for the identification and differentiation of Eimeria species in mixed infections. 用本項研究所建立的特異PCR方法對這些混合感染的球蟲(chóng)種類(lèi)進(jìn)行鑒定具有一定的實(shí)用價(jià)值。
- We have achieved lots of exciting achivements in experting Solar power; different categories of clothing and textile stocks;and the rubber production,such as PCR,TPR,OTR etc. 在各類(lèi)男、女、兒童四季服裝和各類(lèi)庫存紡織品；橡膠制品轎車(chē)胎.;載重胎
- allele specific amplification(ASA) 等位基因特異性擴增
- allele specific oligonucleotide(ASO) 等位基因特異性寡核苷酸, 等位基因特異的
- Using methylation specific PCR (MSP) to detect the CpG island methylation changes of PRA & PRB promoters in the leukemia cell lines before and after 5-Aza CdR treatments and the MG88 transfection. 利用MSP檢測白血病細胞株在5-Aza CdR處理前后、MG88的轉染前后PRA、PRB啟動(dòng)子CpG島甲基化狀態(tài)。
- allele specific oligonucleotide hybridization 等位基因特異寡核苷酸雜交
- If food was cultured for 25 hours for proliferation in listeria enrichment broth, then the culture was chemically extracted, the purified bacteria after heat lysis could react as PCR templates and the inhibitory effects could be greatly reduced. 經(jīng)過(guò)25小時(shí)的增菌培養，對培養物進(jìn)行化學(xué)抽提、純化的菌體經(jīng)加熱裂解后直接用作pcr反應模板，可使抑制作用大大降低。
