Methods The specific PCR primers were designed and synthesized to amplify the DNA fragments enconding preS1/S2/S antigen gene from the plasmid pHBV adr by PCR method.

 
  • 方法設計合成2對寡核苷酸引物,以adr亞型HBV質(zhì)粒pHBV DNA為模板,采用PCR法分別擴增HBV大分子表面蛋白基因(preS1/S2/S基因)片段;
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