If food was cultured for 25 hours for proliferation in listeria enrichment broth, then the culture was chemically extracted, the purified bacteria after heat lysis could react as PCR templates and the inhibitory effects could be greatly reduced.
英
美
- 經(jīng)過(guò)25小時(shí)的增菌培養，對培養物進(jìn)行化學(xué)抽提、純化的菌體經(jīng)加熱裂解后直接用作pcr反應模板，可使抑制作用大大降低。
