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- 人類(lèi)端粒酶反轉錄酶human telmerase reverase transcriptase
- 合成的方案是采用先合成AGD、CGG和DAG三個(gè)三核苷二磷酸片段,然后再以AGD+pCGGDAG(3+p6)或AGDCGG+pDAG(6+p3)兩種方式用T_4 RNA連接酶連接成為所需要的九核苷酸。As the first step, three trinucleoside diphosphate fragments AGD, CGG and DAG were synthesized and then, in the presence of T_4 RNA ligase, joined to form the desired nonamer either through route 3+ p6 (AGD+ pCGGDAG) or route 6+p3 (AGDCGG+pDAG). The phosphodiester methed was used in the syntheses of CGG and DAG;
- 用反轉錄PCR(RT-PCR)檢測轉染細胞ATF基因的表達;Then the expression of ATF was analyzed by means of reverse transcription polymerase chain reaction (RT-PCR).
- RNA連接酶反轉錄PCRRNA ligase reverse transcrip-tion-PCR
- 反anti-
- 評價(jià)反轉錄多聚酶鏈反應檢測大腸癌患者血清人端粒酶反轉錄酶mRNA表達的敏感性及特異性:對照組比較Sensitivity and specificity of human telomerase reverse transcriptase mRNA expression in the serum of patients with colorectal carcinoma: a controlled comparison using reverse transcription polymerase chain reaction
- 泛素連接酶ubiquitin ligase, ubiquitinligating enzyme
- 巢式反轉錄PCRNested RT-PCR
- 輔酶A連接酶CoA ligase
- 端粒酶反義核酸telomerase antisensenucleic acids
- 泛素連接酶E3Ubiquitin ligase E3
- 熒光定量反轉錄PCRFluorescence quantitative reverse transcription PCR (FQ RT-PCR)
- 碳氮連接酶類(lèi)Carbon-nitrogen ligases
- 多聚半乳糖醛酸酶反義基因anti-polygalacturonase gene
- α半定量反轉錄PCRSemi-quantitative RT-PCR
- E3泛素連接酶E3 ligase
- 端粒酶反義寡聚脫氧核苷酸telomerase antisense oligodeoxy nucleotides
- 連接酶合成產(chǎn)物的瞬時(shí)速度與細胞中可利用的ATP含量成比例。The degree of bond formation by ligases is proportional to the amount of ATP available in the cell at a particular instant.
- 豬內源性反轉錄病毒5'端非編碼區的克隆及結構分析Cloning of Porcine Endogenous Retrovirus 5'-Untranslated Region and Analysis of its Structure
- 反轉錄PCRreverse transcriptional PCR; RT-PCR