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- α半定量反轉錄PCRSemi-quantitative RT-PCR
- 方法 :取活動(dòng)期LN患者PBMC培養 ,分別以川芎嗪、地塞米松和川芎嗪 +地塞米松進(jìn)行處理 ,以PBS對照 ,采用半定量反轉錄PCR的方法 ,檢測PBMC的B7- 1和B7- 2mR NA表達。Methods: PBMCs from active stage LN were cultivated and treated with PBS, ligustrazin, dexamethasone and ligustrazin plus dexamethasone injection respectively for 3 days. After cultivation, B7-1(CD80)and B7-2 (CD86)mRNA expressions were measured by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR).
- 收集轉染前、轉染后24 h和48 h的毛滴蟲(chóng)細胞,用半定量反轉錄-PCR(RT-PCR)檢測Prx和TrxR的mRNA水平;The levels of Prx and TrxR mRNA were determined 24 h and 48 h post,transfection by relative quantitative RT,PCR,and the growth of Trichomonas vaginalis was estimated under microscope 36 h post,transfection.
- 熒光定量反轉錄PCRFluorescence quantitative reverse transcription PCR (FQ RT-PCR)
- 半semi-
- 半年half a year
- 熒光定量反轉錄聚合酶鏈式反應fluorascent quantitutied reverse transcription polymerase chain reaction
- 半天half of the day
- 半小時(shí)halfhour
- 半角DBC case
- 反恐anti-terrorism
- 轉錄transcribe
- 半月half-moon
- 反義antonymy
- 半場(chǎng)half-court
- 逆反ob-
- 半透明translucent
- 半個(gè)half
- 本實(shí)驗運用了Marker Rescue Assay(補救分析)和RT/PCR(逆轉錄PCR)兩種方法。In this study, two methods, namely the marker rescue assay and RT/PCR, were employed to detect RCR in the cells.
- 半路midway
