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- C DNA模板擴增。c and the DNA template of p.
- 含有人全長(cháng)CblcDNA的質(zhì)粒pEFHACbl作為模板擴增Cbl基因N-端(CblN);The gene fragment encoding the N-terminal part of Cbl protein (named as CblN) was amplified by PCR using pEFHACbl plasmid encoding human Cbl as templates.
- 轉錄酶一種在轉錄過(guò)程中催化DNA模板形成RNA的聚合酶A polymerase that catalyzes the formation of RNA from a DNA template in the process of transcription.
- 采用RT PCR技術(shù) ,以HL 60細胞mRNA為模板擴增獲得編碼BPI氨基端 1 93個(gè)氨基酸 (rBPI2 1)的基因片段 (BPI60 0 ) ;BPI 600 bp gene which encode 193 amino acid fragments (rBPI 21 ) BPI N terminal was amplified by RT PCR from mRNA that were extracted from HL 60;
- PCR方法檢測轉基因食品時(shí),應用改良CTAB法制備DNA模板。DNA extract by using modified CTAB methods was suitable for PCR-based detection of genetically modified organisms in foods and animals feed.
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- 擴增augmentation
- 對5種轉基因棉籽和3種非轉基因棉籽,應用改良CTAB法和改良KIT法提取DNA模板,對35S、NOS、Bt等基因進(jìn)行了檢測研究,并確定了在檢測Bt基因時(shí)的靈敏度。The study of detection of 35S? NOS? Bt in 5 transgenic and 3 non-transgenic cotton seeds was carried out with DNA extraction by means of modified CTAB method and modified kit method respectively.
- 方法以質(zhì)粒pEGFP-N1為模板擴增EGFP基因,EcoR I和Hind III雙酶切EGFP基因序列和pET28a載體。Methods Using plasmid as template,full-length EGFP gene was amplified. The amplified EGFP gene and pET28a vector were cleaved by EcoR I and Hind III,then ligated after purifying.
- 人腦原發(fā)性腫瘤中c-fos基因的擴增、重排及過(guò)強表達AMPLIFICATION,REARRANGEMENT AND OVER-EXPRESSION OF c-fos GENE IN HUMAN PRIMARY BRAIN TUMORS
- 擴增性Proliferation
- 擴增圖譜amplification map
- cDNA擴增cDNA amplification
- 擴增條帶Amplified band
- 種質(zhì)擴增Enhancement
- 分段擴增PCR
- RAPD擴增RAPD
- 擴增突變trinucleotide repeat expansion
- 擴增和缺失expansion and contraction instability
- 單管PCR擴增one tube PCR amplification
