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- RAPD擴增RAPD
- 以改進(jìn)的CTAB法獲得了較高質(zhì)量的胡枝子屬植物基因組DNA，可以很好地用于RAPD擴增研究。With the improved CTAB protocol, high quality genome DNA of the Lespedeza populations were obtained for RAPD amplification.
- 說(shuō)明改良的CTAB法能成功用于梨屬植物DNA提取,建立的最佳反應體系可用于梨屬植物RAPD擴增。It explained that reformed CTAB method could extract the genomic DNA of Pyrus successfully,and the optimum RAPD reaction system could amplificate RAPD in Pyrus L..
- 結果表明,在RAPD擴增中存在著(zhù)大量的錯配,這些錯配的產(chǎn)生有很大的隨機性,這使擴增中錯配結合的方式很多;Elena took a series experiments of RAPD to study the kind of the mismatch.
- 應用44種隨機引物對10種麥紅吸漿蟲(chóng)滯育蟲(chóng)態(tài)進(jìn)行RAPD擴增,共篩選出6條隨機引物S1,S50,S81,S83,S1031,S1248可以對10種麥紅吸漿蟲(chóng)滯育蟲(chóng)態(tài)擴增出穩定清晰的多態(tài)性片斷(附圖1)。The genomic DNA of ten samples was amplified with 44 random primers. Only six primers (S1, S50, S81, S83, S1031, S1248) could produce clear fragments and obtained a total number of 81 fragments in ten samples amplified (Fig.1).
- 擴增性Proliferation
- 結果表明除SDS法外,其余兩種方法均可獲得完整的海帶基因組DNA,RAPD擴增譜帶清晰穩定。 尤以改良CTAB法提取的DNA質(zhì)量較好。The result was that the latter two methods could be used for extracting genomic DNA from sporophyte of Laminaria, and that the improved CTAB method was the best ones through which the production had the highest purity.
- 擴增圖譜amplification map
- cDNA擴增cDNA amplification
- 擴增條帶Amplified band
- 種質(zhì)擴增Enhancement
- 分段擴增PCR
- 擴增突變trinucleotide repeat expansion
- 酶（促）擴增enzymatic amplification
- 擴增和缺失expansion and contraction instability
- 單管PCR擴增one tube PCR amplification
- 干細胞擴增stem cell expansion
- PCR擴增條件PCR amplification conditions
- 選擇性擴增Selective amplification
- 多重耐藥株以及敏感株acrAB基因擴增產(chǎn)物無(wú)點(diǎn)突變和缺失。No deletion or point mutation in acrAB gene were found in Mar and susceptible clinical Escherichia coli isolates.
