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- 長(cháng)距離反向PCRlong distance-inverse PCR
- 巢式反向PCRnested inverse PCR
- 長(cháng)距離反向散射long-distance-back scatter
- 采用反向PCR技術(shù)獲得了該基因的編碼區克隆。 分別構建含有內含子和不含內含子的AVA基因植物表達載體pBI121AVA-GUS和pBI121AVAc-GUS,通過(guò)根癌農桿菌介導法轉化煙草。The AVA gene with and without intron were inserted downstream of 35S promoter into a vetor pBI121 to get pBI121AVA-GUS and pBI121AVAc-GUS vetor respectively.
- 方法在已成功克隆和表達人HMGB1的基礎上,采用一步反向PCR致突變法構建人HMGB1cDNA的6個(gè)突變體,并分別克隆于原核表達載體pQE-80L上,表達6個(gè)相應的重組HMGB1突變體蛋白,并經(jīng)Western blot鑒定。Methods This experiment was based on successful clone and expression of human HMGB1.Six mutant HMGB1 cDNA were designed and constructed by one step inverse PCR. They were cloned into prokaryotic expressive vector pQE80L and followed with production of mutant HMGB1s and identification by Western blotting.
- PCRpolymerase chain reaction (PCR)
- 反向輻射狀免疫擴散reverse radial immunodiffusion ( RRID)
- PCR法PCR
- 作用于磁化材料以去除剩磁的反向磁通強度。The opposing magnetic intensity that must be applied to a magnetized material to remove the residual magnetism.
- 3-PCR3-PCR
- 反向遞減reverse regression
- TD-PCRTD-PCR
- 棉條反向sliver reversal
- UP-PCRUP-PCR
- 反向旋轉減少了凈摩擦力。The counterrotation reduces the net friction torque.
- 組合PCRassembly PCR
- 集電結是反向編置。The collector junction biased in the reverse direction.
- 搭橋PCRbridge-PCR
- 反向帶寬inverse bandwidth
- 熒光PCRfluorescent PCR
