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- 巢式反向PCRnested inverse PCR
- PCR-流過(guò)式反向斑點(diǎn)雜交PCR-flow through reverse dot blot
- 壓緊式反向接頭imapct reverse sub
- 長(cháng)距離反向PCRlong distance-inverse PCR
- 巢式PCRNested PCR
- 嵌入式flush bonding
- 巢式nesting
- 采用反向PCR技術(shù)獲得了該基因的編碼區克隆。 分別構建含有內含子和不含內含子的AVA基因植物表達載體pBI121AVA-GUS和pBI121AVAc-GUS,通過(guò)根癌農桿菌介導法轉化煙草。The AVA gene with and without intron were inserted downstream of 35S promoter into a vetor pBI121 to get pBI121AVA-GUS and pBI121AVAc-GUS vetor respectively.
- 式的fashioned
- 巢式窖[化] beehive kiln
- 美式American
- 式中wherein
- 方法在已成功克隆和表達人HMGB1的基礎上,采用一步反向PCR致突變法構建人HMGB1cDNA的6個(gè)突變體,并分別克隆于原核表達載體pQE-80L上,表達6個(gè)相應的重組HMGB1突變體蛋白,并經(jīng)Western blot鑒定。Methods This experiment was based on successful clone and expression of human HMGB1.Six mutant HMGB1 cDNA were designed and constructed by one step inverse PCR. They were cloned into prokaryotic expressive vector pQE80L and followed with production of mutant HMGB1s and identification by Western blotting.
- 美式英語(yǔ)American English
- 嵌入式系統embedded system
- 巢床PCRnested PCR
- 日式Japanese
- 單式simplex
- 巴洛克式barpque
- 菜式cuisine
