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- 重疊延伸技術(shù)（SOE）splicing by overlap extension (SOE)
- PCR重疊延伸技術(shù)splicing by overlap extension
- 方法擴增EGFP及TK并采用重疊延伸拼接(SOE)法連接EGFP和TK,將EGFP-TK融合基因亞克隆至pBABE-puro-lox的Sal I和Cla I位點(diǎn)之間,構建成新載體pBGTKlox。METHODS:EGFP and TK were amplified by polymerase chain reaction and fused by splicing overlap extension. The fusion gene EGFP-TK was then subcloned to the sites Sal I and Cla I of pBABE-puro-lox to construct pBTKlox.
- 延伸技術(shù)Elongation technique
- 擴增前的引物延伸技術(shù)primer extension preamplification; PEP
- 重疊延伸PCRoverlap extension PCR
- 重疊延伸PCR法overlap extension by polymerase chain reaction
- SOE法SOE
- 重疊overlapping
- 幾種分散控制系統的SOE性能測試比較Contrast on the Result of SOE Performance Tests of Several DCS
- DCS系統SOE性能的測試及分析Test and analysis of SOE performance in DCS
- 重疊延伸overlap extension
- 同時(shí)對光纖差動(dòng)保護運用于線(xiàn)路變壓器組時(shí)涉及的矢量變換、勵磁涌流、TA飽和等延伸技術(shù)進(jìn)行了分析，解決了傳統的線(xiàn)路光纖差動(dòng)保護不能滿(mǎn)足線(xiàn)路變壓器組保護的要求。The extending techniques such as vector transform, excitation inrush current , TA saturation and so on are analyzed, which resolves the question that the traditional optical differential protection can not satisfy the request of line-transformer unit.
- 重疊延伸拼接法splicing overlap extension; SOE
- SOE事件記錄:在線(xiàn)記錄事件量達64條，先進(jìn)先出（IFO）態(tài)刷新，帶有時(shí)間標記，掉電不丟失。SOE event record: can record up to64 online events, FIFO dynamic refresh, possess time mark, no lose when power supply drop.
- 我們缺少技術(shù)工人。We are short of skilled labor.
- 群山向西延伸。The mountains have a western trend.
- 重疊的overlapped
- SOE事件記錄:在線(xiàn)記錄事件量達64條，先進(jìn)先出（FIFO）動(dòng)態(tài)刷新，帶有時(shí)間標記，掉電不丟失。SOE event record: can record up to 64 online events, FIFO dynamic refresh, possess time mark, no lose when power supply drop.
- 六年的待業(yè)并未使他的技術(shù)荒疏。A six-year lay-off has not rusted his skills.
